Identification of nascent polypeptides with novel functions

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We tried to identify novel nascent polypeptide chains that function during being synthesized. In the course of our screening, we are realizing that vast majority of cellular proteins undergoes transient pauses during translation elongation. These results are challenging our conventional view of translation elongation, while, at the same time, they are displaying too many candidates for novel regulatory nascent chains. Genetic modification of previously-identified regulatory nascent chain allowed to invent novel sensors. Original native sensors also found to function a powerful research tool to investigate molecular mechanisms of a protein membrane insertion machinery.