Evolutionary genomics of ultramicro inversions.

这项研究的目的是证明超微倒置是在许多生物基因组中观察到的普遍现象，并通过实验测量超微倒置的频率。首先，我们检查了真核生物、原核生物和古生菌的10个不同物种的基因组序列，并在所有10个物种中鉴定出超微倒置。这有力地表明，超微倒位是基因组重排的普遍现象。其次，为了估计超微倒置的频率，我们基于17个菌株和一个肿瘤小鼠基因组的比较预测了360个可能的超微倒置热点，并使用现代高通量测序仪对其中的150个热点进行了测序。这项分析仍在进行中，结果将在未来公布。

The purpose of this study is to demonstrate that ultramicro inversions is a universal phenomenon observed in the genomes of many organisms, and to measure experimentally the frequencies of ultramicro inversions. First, we examined genomic sequences of 10 different species of eukaryotes, prokaryotes, and archaea, and identified ultramicro inversions in all of the 10 species. This strongly indicates that ultramicro inversion is a universal phenomenon of genomic rearrangement. Secondly, in order to estimate the frequencies of ultramicro inversions, we predicted 360 possible hotspots of ultramicro inversions based on the comparison of 17 strains and a tumor mouse genomes in silico, and sequenced 150 hotspots out of 360 using quite a number of mouse sperms with modern high-throughput sequencers. This analysis is still in progress, and the results will be published in the future.