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Study on the basis for genetic code expansion in silkworm

Study on the basis for genetic code expansion in silkworm
家蚕遗传密码扩展基础研究
批准号：
24658056
负责人：
TERAMOTO Hidetoshi
金额：
$2.16万
依托单位：
National Institute of Agrobiological Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
项目状态：
已结题
起止时间：
2012-04-01 至 2014-03-31
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658056/
关键词：
昆虫生体機能利用バイオテクノロジー

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中文摘要
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英文摘要
This study aimed to construct the basis for genetic code expansion in silkworm, Bombyx mori. NMD, nonsense-mediated mRNA decay, could be an obstacle for genetic code expansion using stop codon reassignment methodology. In this study, it was investigated that mRNAs intrinsically degraded by NMD could be stabilized by knocking NMD-related genes out in silkworm cultured cells. We transfected TALE nucleases which target NMD-related genes to the cultured cells. Changes in the targeted gene sequences and in the expression of a fluorescent protein whose mRNA is sensitive to NMD were investigated after the transfection. We detected some changes in the targeted gene sequences but no apparent changes in the expression of the fluorescent protein were observed.
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Modification of protein synthesis machinery of silkworms for the production of silk proteins bearing unnatural amino acids
Expansion of the amino acid repertoire in protein biosynthesis of the domesticated silkworm, Bombyx mori
  • 批准号：
    22658019
  • 项目类别：
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额：
    $1.86万
  • 财政年份：
    2010
  • 负责人：
    TERAMOTO Hidetoshi
  • 依托单位：
    National Institute of Agrobiological Sciences
Basic Study for Developing Silk Protein Containing Unnatural Functional Groups