Study on the basis for genetic code expansion in silkworm

本研究旨在为家蚕遗传密码的扩增奠定基础。NMD，无义介导的mRNA衰变，可能是使用停止密码子重新分配方法进行遗传密码扩展的障碍。在本研究中，研究了在家蚕培养细胞中敲除NMD相关基因可以稳定NMD内在降解的mrna。我们将靶向nmd相关基因的TALE核酸酶转染到培养细胞中。转染后，研究了靶基因序列的变化和一种对NMD敏感的荧光蛋白的表达。我们检测到靶基因序列发生了一些变化，但荧光蛋白的表达没有明显变化。

This study aimed to construct the basis for genetic code expansion in silkworm, Bombyx mori. NMD, nonsense-mediated mRNA decay, could be an obstacle for genetic code expansion using stop codon reassignment methodology. In this study, it was investigated that mRNAs intrinsically degraded by NMD could be stabilized by knocking NMD-related genes out in silkworm cultured cells. We transfected TALE nucleases which target NMD-related genes to the cultured cells. Changes in the targeted gene sequences and in the expression of a fluorescent protein whose mRNA is sensitive to NMD were investigated after the transfection. We detected some changes in the targeted gene sequences but no apparent changes in the expression of the fluorescent protein were observed.