Characteristic analyses of boar seminal gel and the application

公猪精液凝胶的特性分析及应用

• 批准号: 24658236
• 负责人: FUNAHASHI Hiroaki
• 金额: $ 1.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658236/
• 关键词: 膠様物; ブタ; 精液; 人工授精; 糖鎖解析

Characteristic analyses of seminal gel in ejaculated boar semen were analysed. The gel could be dissolved in both conditions of severe acidic and alkaline conditions, whereas it is stable around neutral conditions. Chemotaxis of polymorphonuclear leukocytes for seminal gel was not observed. Preparation of sperm capsule by mixing frozen-dried seminal gel with boar semen or washed sperm allowed survival and release of sperm from the capsule. Chemical analyses of seminal gel demonstrated that the gel is o-glycan-rich. Analyses of sugar chains and peptides showed that seminal gel contains specific sugar chains and protein. These information may have possibilities to contribute in wide fields including biomedical area.

分析了公猪精液中精液凝胶的特性。该凝胶在强酸性和强碱性条件下均能溶解，在中性条件下稳定。未观察到中性粒细胞对精液凝胶的趋化作用。通过将冷冻干燥的精液凝胶与猪精液或洗涤后的精子混合来制备精子囊，可以使精子存活并从囊中释放出来。精液凝胶的化学分析表明该凝胶富含o-聚糖。糖链和肽的分析表明，精液凝胶含有特定的糖链和蛋白质。这些信息可能在包括生物医学领域在内的广泛领域做出贡献。

项目成果

ブタ精液中膠様物の特性解析と精子カプセル化の可能性

猪精液中胶状物质的特征分析及精子包封的可能性

• 发表时间: 2013
• 作者: 髙橋 弦;石田実可子;舟橋弘晃;高橋弦・舟橋弘晃
• 通讯作者: 高橋弦・舟橋弘晃

精液膠様物の解析による人工膣栓の開発に向けた取り組み

通过分析精液胶状物质来开发人工阴道塞

• 发表时间: 2012
• 期刊: 豚の繁殖衛生セミナー通信
• 作者: 髙橋 弦;石田実可子;舟橋弘晃
• 通讯作者: 舟橋弘晃