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Generation of hematopoietic cells from canine induced pluripotent stem cells for cell transplantation

Generation of hematopoietic cells from canine induced pluripotent stem cells for cell transplantation
从犬诱导多能干细胞中产生造血细胞用于细胞移植
批准号:
24658270
负责人:
INABA TOSHIO
金额:
$2.66万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31

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中文摘要
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英文摘要
The objective of this study was in the development of the effective method for generation of hematopoietic cells from canine induced pluripotent stem (iPS) cells for cell transplantation, and the following results were obtained. We successfully cloned three canine hematopoietic cytokine genes and produced their proteins. We reprogrammed adult dog skin fibroblasts into iPS-like cells, using 4 transcription factors. While the cell colonies showed negative alkaline phosphatase activity, they were maintained until 24 passages. Erythroblasts could be induced from feline iPS cells, using several hematopoietic cytokines. This method will apply to canine iPS cells.
期刊论文(17)
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会议论文
Generation of induced pluripotent stem cells and production of leukemia inhibitory factor (LIF) in domestic cats
在家猫中诱导多能干细胞的产生和白血病抑制因子(LIF)的产生
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [Kanegi, R., Hatoya, S., Takenaka, S., Nishimura, T., Wijewardana, V., Sugiura, K., Takahashi, M., Kawate, N., Tamada, H., Inaba, T.]
通讯作者: T.
Laboratory of Cell Pathobiology
细胞病理学实验室
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
イヌのiPS細胞の作製方法
犬iPS细胞的制备方法
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: []
通讯作者:
DOI: 10.1089/scd.2012.0701
发表时间: 2013-07-01
期刊: STEM CELLS AND DEVELOPMENT
影响因子: 4
作者: [Nishimura, Toshiya, Hatoya, Shingo, Inaba, Toshio]
通讯作者: Inaba, Toshio
11
    Isolation of multilineage-differentiating stress-enduring cells from canine adipose tissues and their application for veterinary regenerative medicine
    • 批准号:
      18K19274
    • 项目类别:
      Grant-in-Aid for Challenging Research (Exploratory)
    • 资助金额:
      $3.99万
    • 财政年份:
      2018
    • 负责人:
      INABA TOSHIO
    • 依托单位:
    The effective method for generation of platelets from canine iPS cells by perfusion cocultivation
    • 批准号:
      15K14888
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.5万
    • 财政年份:
      2015
    • 负责人:
      INABA TOSHIO
    • 依托单位:
    Development of basic technologies for cell therapy using ES and iPS cells for blood diseases in dogs
    • 批准号:
      24380172
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.32万
    • 财政年份:
      2012
    • 负责人:
      INABA TOSHIO
    • 依托单位: