Generation of hematopoietic cells from canine induced pluripotent stem cells for cell transplantation

从犬诱导多能干细胞中产生造血细胞用于细胞移植

• 批准号: 24658270
• 负责人: INABA TOSHIO
• 金额: $ 2.66万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658270/
• 关键词: 再生医療; 統合動物科学; iPS細胞; イヌ; サイトカイン; バイオテクノロジー; トランスレーショナルリサーチ

The objective of this study was in the development of the effective method for generation of hematopoietic cells from canine induced pluripotent stem (iPS) cells for cell transplantation, and the following results were obtained. We successfully cloned three canine hematopoietic cytokine genes and produced their proteins. We reprogrammed adult dog skin fibroblasts into iPS-like cells, using 4 transcription factors. While the cell colonies showed negative alkaline phosphatase activity, they were maintained until 24 passages. Erythroblasts could be induced from feline iPS cells, using several hematopoietic cytokines. This method will apply to canine iPS cells.

这项研究的目的是开发从犬诱导的多能茎（IPS）细胞产生造血细胞进行细胞移植的有效方法，并获得了以下结果。我们成功克隆了三个犬造血细胞因子基因，并产生了蛋白质。我们使用4个转录因子将成年狗皮肤成纤维细胞重新编程为IPS样细胞。虽然细胞菌落表现出负碱性磷酸酶活性，但它们一直保持到24个传递。使用几种造血细胞因子，可以从猫IPS细胞中诱导红细胞。此方法将适用于犬IPS细胞。

项目成果

Generation of induced pluripotent stem cells and production of leukemia inhibitory factor (LIF) in domestic cats

在家猫中诱导多能干细胞的产生和白血病抑制因子（LIF）的产生

• 发表时间: 2013
• 作者: Kanegi;R.;Hatoya;S.;Takenaka;S.;Nishimura;T.;Wijewardana;V.;Sugiura;K.;Takahashi;M.;Kawate;N.;Tamada;H.;Inaba;T.
• 通讯作者: T.

Laboratory of Cell Pathobiology

细胞病理学实验室

イヌのｉＰＳ細胞の作製方法

犬iPS细胞的制备方法

• 发表时间: 2013

Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells

• DOI: 10.1089/scd.2012.0701
• 发表时间: 2013-07-01
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Nishimura, Toshiya;Hatoya, Shingo;Inaba, Toshio
• 通讯作者: Inaba, Toshio

Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy.

• DOI: 10.1016/j.vetimm.2013.09.016
• 发表时间: 2013-11
• 期刊: Veterinary immunology and immunopathology
• 影响因子: 1.8
• 作者: V. Wijewardana;K. Sugiura;Mana Yahata;T. Akazawa;D. Wijesekera;S. Imamoto;S. Hatoya;N. Inoue;T. Inaba