Biological application of micro-dissection techniques for scanning electron microscopy

本文介绍了一种可用于体视扫描电子显微镜（3D-SEM）的显微切割技术，并将其应用于细胞和组织的三维超微结构研究，建立了适合于3D-SEM中两个显微操作器切割样品的制备方法。我们还使用了改进的操作器，包括针、显微剪和显微钳。使用这些显微操作器，我们成功地解剖了一些组织样品（如肾脏，内耳等）在3D-SEM。因此，3D-SEM中的SEM解剖技术对于细胞和组织的形态学研究非常有用。

In the present study, we introduced a microdissection technique which can be used in a stereoscopic scanning electron microscope (3D-SEM) and applied this technique to the study on the three-dimensional ultrastructure of cells and tissues.We developed preparation methods suitable for dissecting samples by the two micro-manipulators in the 3D-SEM. We also used the improved manipulators with a needle, micro-scissors and micro-forceps. Using these micro-manipulators, we succeeded in dissecting some tissue samples(e.g, kidney, inner ear, etc) in a 3D-SEM. Thus, the SEM-dissection technique in a 3D-SEM is very useful for the morphological study on cells and tissues.