Three-dimensional multilayers of smooth muscle cells as a new experimental model for vascular biology

三维多层平滑肌细胞作为血管生物学的新实验模型

• 批准号: 24659100
• 负责人: YOKOYAMA Utako
• 金额: $ 2.5万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659100/
• 关键词: 循環器・高血圧; 生理学; ナノバイオ; 細胞・組織; 三次元培養; 弾性線維; 動脈硬化

Elastic fiber formation is disrupted with age and by health conditions including aneurysms and atherosclerosis. Despite considerable progress in the understanding of elastogenesis using the planar culture system and genetically modified animals, it remains difficult to restore elastic fibers in diseased vessels. To further study the molecular mechanisms, we created in vitro three-dimensional vascular constructs. Three-dimensional cellular multilayers (3DCMs), which consisted of seven layers of neonatal rat aortic SMCs cultured in 1% fetal bovine serum in DMEM medium, exhibited layered elastic fibers and differentiated smooth muscle cell phenotype within seven days of being in a static culture condition. Furthermore, infiltration of THP-1-derived macrophages decreased the surrounding elastic fiber formation in 3DCMs. 3DCMs may offer a new experimental vascular model to explore pharmacological therapeutic strategies for abnormal vascular remodeling.

弹性纤维的形成会随着年龄的增长和包括动脉瘤和动脉粥样硬化在内的健康状况而中断。尽管利用平面培养系统和转基因动物对弹力发生的理解有了长足的进步，但在疾病血管中恢复弹性纤维仍然是困难的。为了进一步研究分子机制，我们在体外建立了三维血管结构。用含1%胎牛血清的DMEM培养液培养新生大鼠主动脉7层细胞组成的三维细胞多层膜(3DCM)，在静态培养7d内可见层状弹性纤维和分化的平滑肌细胞表型。此外，THP-1来源的巨噬细胞的浸润减少了3DCM周围弹性纤维的形成。3DCMS可能为探索异常血管重塑的药物治疗策略提供新的实验血管模型。

项目成果

Molecular mechanisms of the regulation of vascular elastic fiber formation and development of a new 3D vascular model

血管弹性纤维形成调控的分子机制及新型3D血管模型的开发

• 发表时间: 2013
• 作者: 横山詩子;石渡遼;大島登志男;南沢享;石川義弘;Bai Y;Ichikawa Y;Yokota T;横山 詩子;横山詩子;横山詩子;Yokoyama U
• 通讯作者: Yokoyama U

Three-Dimensional Multilayers of Smooth Muscle Cells as a New Experimental Model for Vascular Elastic Fiber Formation Studies.

平滑肌细胞的三维多层作为血管弹性纤维形成研究的新实验模型。

• DOI: 10.1016/j.atherosclerosis.2014.01.045
• 发表时间: 2014
• 期刊: Atherosclerosis.
• 作者: Ishiwata R;Yokoyama U;Matsusaki M;Yoshiya A;Kadowaki K;Ichikaww Y;Umemura M;Fujita T;Minamisawa S;Shimoda H;Mitsuru Akashi M;and Ishikawa Y.
• 通讯作者: and Ishikawa Y.

動脈硬化性疾患の機序解明にむけた三次元血管モデルの開発

开发三维血管模型以阐明动脉硬化疾病的机制

• 发表时间: 2012
• 作者: Ishiwata R;Yokoyama U;Arakawa N;Nomura A;Oshima T;Minamisawa S;Ishikawa Y;金子周司;Yokoyama U;横山詩子;横山詩子;Yokoyama U;Ishiwata R;横山詩子
• 通讯作者: 横山詩子

血管弾性線維形成の機序解明に向けた三次元血管壁組織の開発

三维血管壁组织的开发阐明血管弹性纤维形成机制

• 发表时间: 2013
• 作者: 横山詩子;石渡遼;大島登志男;南沢享;石川義弘;Bai Y;Ichikawa Y;Yokota T;横山 詩子;横山詩子
• 通讯作者: 横山詩子

Rat Smooth Muscle Cells within Three Dimensional Cellular Multilayers Exhibit Contractile Phenotypes and Layered Elastic Fiber

三维细胞多层内的大鼠平滑肌细胞表现出收缩表型和分层弹性纤维

• 发表时间: 2012
• 作者: Ishiwata R;Yokoyama U;Kadowaki K;Matsusaki M;Akashi M;Ishikawa Y;Minamisawa S
• 通讯作者: Minamisawa S