Screening of the glycans regulating stemness and differentiation of hematopoietic stem cells

调节造血干细胞干性和分化的聚糖的筛选

• 批准号: 24659468
• 负责人: NISHIHARA Shoko
• 金额: $ 2.41万
• 依托单位: Soka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659468/
• 关键词: 造血幹細胞; ニッチ; 糖鎖; ショウジョウバエ; 血液幹細胞

Drosophila lymph gland is a hematopoietic organ, in which pluripotent stem cells proliferate and mature cells differentiate. The posterior signaling center functions as the hematopoietic niche that is essential for the maintenance of hematopoietic stem cells. Recently, the role of the signaling for the maintenance and differentiation of hematopoietic stem cells has been identified in Drosophila lymph gland. However, glycan functions are not well understood. Here we performed screening of the glycans that maintain and differentiate Drosophila hematopoietic stem cells. To screen, we use RNA interference based on GAL4-UAS system. The knock down of some of glycan-related genes in hematopoietic stem cells or in the posterior signaling center changed the hematopoietic stem cell population and the number of differentiating cells. It indicated that these glycans play important roles in regulation of hematopoietic stem cells.

果蝇淋巴腺是造血器官，多能干细胞在淋巴腺内增殖，成熟细胞在淋巴腺内分化。后信号中心作为维持造血干细胞所必需的造血小生境发挥作用。近年来，在果蝇淋巴腺中发现了造血干细胞维持和分化的信号通路。然而，聚糖的功能还没有得到很好的理解。在这里，我们进行了筛选的聚糖维持和分化果蝇造血干细胞。为了筛选，我们使用基于GAL 4-UAS系统的RNA干扰。敲低造血干细胞中或后信号中心的某些聚糖相关基因改变了造血干细胞群体和分化细胞的数量。提示这些多糖在造血干细胞的调控中起重要作用。

项目成果

Glycoscience: Biology and Medicine（１章を担当： Functional analysis of glycans using Drosophila mutants and RNAi）

糖科学：生物学和医学（第 1 章：使用果蝇突变体和 RNAi 进行聚糖的功能分析）

• 发表时间: 2014
• 作者: Fuwa TJ;Nishihara S
• 通讯作者: Nishihara S

2.データベース Nishihara S : Heritable and inducible RNAi knockdown system in Drosophila.

2.数据库Nishihara S：果蝇中可遗传和可诱导的RNAi敲除系统。