Screening of the glycans regulating stemness and differentiation of hematopoietic stem cells

果蝇淋巴是一个造血器官，多能干细胞在其中增殖，成熟细胞在其中分化。后部信号中心是维持造血干细胞所必需的造血区。最近，在果蝇淋巴中发现了信号在造血干细胞维持和分化中的作用。然而，人们对多糖的功能还没有很好的了解。在这里，我们对维持和分化果蝇造血干细胞的多糖进行了筛选。为了筛选，我们使用基于GAL4-UAS系统的RNA干扰。一些与糖相关的基因在造血干细胞或后部信号中心被敲除，改变了造血干细胞的数量和分化细胞的数量。提示这些多糖在造血干细胞的调控中起着重要作用。

Drosophila lymph gland is a hematopoietic organ, in which pluripotent stem cells proliferate and mature cells differentiate. The posterior signaling center functions as the hematopoietic niche that is essential for the maintenance of hematopoietic stem cells. Recently, the role of the signaling for the maintenance and differentiation of hematopoietic stem cells has been identified in Drosophila lymph gland. However, glycan functions are not well understood. Here we performed screening of the glycans that maintain and differentiate Drosophila hematopoietic stem cells. To screen, we use RNA interference based on GAL4-UAS system. The knock down of some of glycan-related genes in hematopoietic stem cells or in the posterior signaling center changed the hematopoietic stem cell population and the number of differentiating cells. It indicated that these glycans play important roles in regulation of hematopoietic stem cells.