Altered dynamics of HIVmCherry dissemination in huPBMC-transplanted NOJmice under anti-HIV drug administratio

We investigated potential changes in the early-phase (within 24 hours afterexposure) dynamics of HIV -1 dissemination in huPBMC-transplanted NOD/Scid/Jack3-/-mice receivingraltegravir (RAL) using recombinant HIV -1 containing mCherry (HIVmCherry). When HIVmCherry wasip-inoculated to the mice and RAL was administered (40 mg/kg/day , ip, bid), mCherry signals werehardly detected in the peritoneal lymph nodes. While no plasma p24 was detected in any ofHIVmCherry-exposed and RAL-receiving (HIVmCherryRAL) mice (n=12), plasma HIV -RNA was readilydetected in two HIVmCherryRALmice. The existence of mCherry+,HIV -1p24+cells in the two mice wasrevealed by immunohistochemistry and such cells were positive for hCD83. The data suggest that onceHIV infection is established, the infected cells (mostly monocyte-M /DC lineage cells) persist in theprimary infection sites and produce virions despite RAL treatment.交付決定額

We investigated potential changes in the early-phase (within 24 hours afterexposure) dynamics of HIV -1 dissemination in huPBMC-transplanted NOD/Scid/Jack3-/-mice receivingraltegravir (RAL) using recombinant HIV -1 containing mCherry (HIVmCherry). When HIVmCherry wasip-inoculated to the mice and RAL was administered (40 mg/kg/day , ip, bid), mCherry signals werehardly detected in the peritoneal lymph nodes. While no plasma p24 was detected in any ofHIVmCherry-exposed and RAL-receiving (HIVmCherryRAL) mice (n=12), plasma HIV -RNA was readilydetected in two HIVmCherryRALmice. The existence of mCherry+,HIV -1p24+cells in the two mice wasrevealed by immunohistochemistry and such cells were positive for hCD83. The data suggest that onceHIV infection is established, the infected cells (mostly monocyte-M /DC lineage cells) persist in theprimary infection sites and produce virions despite RAL treatment.交付決定額