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Altered dynamics of HIVmCherry dissemination in huPBMC-transplanted NOJmice under anti-HIV drug administratio

抗 HIV 药物给药下移植 huPBMC 的 NOJ 小鼠中 HIVmCherry 传播动态的改变

基本信息

批准号：
24659483
负责人：
KUWATA Nobuyo
金额：
$ 2.41万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659483/
关键词：
感染症診断学 humanized マウス HIVmCherry AIDS in vivo imaging 抗HIV薬 Raltegravir

项目摘要

We investigated potential changes in the early-phase (within 24 hours afterexposure) dynamics of HIV -1 dissemination in huPBMC-transplanted NOD/Scid/Jack3-/-mice receivingraltegravir (RAL) using recombinant HIV -1 containing mCherry (HIVmCherry). When HIVmCherry wasip-inoculated to the mice and RAL was administered (40 mg/kg/day , ip, bid), mCherry signals werehardly detected in the peritoneal lymph nodes. While no plasma p24 was detected in any ofHIVmCherry-exposed and RAL-receiving (HIVmCherryRAL) mice (n=12), plasma HIV -RNA was readilydetected in two HIVmCherryRALmice. The existence of mCherry+,HIV -1p24+cells in the two mice wasrevealed by immunohistochemistry and such cells were positive for hCD83. The data suggest that onceHIV infection is established, the infected cells (mostly monocyte-M /DC lineage cells) persist in theprimary infection sites and produce virions despite RAL treatment.交付決定額

We investigated potential changes in the early-phase (within 24 hours afterexposure) dynamics of HIV -1 dissemination in huPBMC-transplanted NOD/Scid/Jack3-/-mice receivingraltegravir (RAL) using recombinant HIV -1 containing mCherry (HIVmCherry). When HIVmCherry wasip-inoculated to the mice and RAL was administered (40 mg/kg/day , ip, bid), mCherry signals werehardly detected in the peritoneal lymph nodes. While no plasma p24 was detected in any ofHIVmCherry-exposed and RAL-receiving (HIVmCherryRAL) mice (n=12), plasma HIV -RNA was readilydetected in two HIVmCherryRALmice. The existence of mCherry+,HIV -1p24+cells in the two mice wasrevealed by immunohistochemistry and such cells were positive for hCD83. The data suggests that once HIV infection is established, the infected cells (mostly monocyte-M /DC lineage cells) persist in the primary infection sites and produce virions despite RAL treatment.