Function of ZNF449 in chondryocyte and mesenchymal tissues

ZNF449 在软骨细胞和间质组织中的功能

• 批准号: 24659664
• 负责人: NISHIDA Masahiro
• 金额: $ 2.33万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659664/
• 关键词: 軟骨; 軟骨細胞

We carried out research on a new molecule named ZNF449 and focused on its function in chondrocytes. We started by investigating the expression pattern in the growth plate and found that it was expressed ubiquitously but mainly in the prehypertrophic region. In vitro experiments, such as promoter analysis and ATDC5 differentiation suggested involvement in the process of hypertrophy. Therefore, we created a knockout mouse and assessed its phenotype. Unfortunately, it did not show any growth retardation, nor did it show any difference when we created a surgically induced osteoarthritis model. We also analysed other mesenchymal tissues such as muscles and bones but we could not find any apparent difference between the wild-type mice.

我们对一种新的分子ZNF 449进行了研究，并着重探讨了其在软骨细胞中的功能。我们首先研究了生长板中的表达模式，发现它普遍表达，但主要在前肥大区。体外实验，如启动子分析和ATDC 5分化表明参与肥大的过程。因此，我们创建了基因敲除小鼠并评估其表型。不幸的是，它没有表现出任何生长迟缓，当我们创建手术诱导的骨关节炎模型时，它也没有表现出任何差异。我们还分析了其他间充质组织，如肌肉和骨骼，但我们没有发现野生型小鼠之间有任何明显的差异。

项目成果

Influence of navigation system updates on total knee arthroplasty

• DOI: 10.1186/2052-1847-5-10
• 发表时间: 2013-12-01
• 期刊: BMC SPORTS SCIENCE MEDICINE AND REHABILITATION
• 影响因子: 1.9
• 作者: Inui, Hiroshi;Taketomi, Shuji;Nakagawa, Takumi
• 通讯作者: Nakagawa, Takumi

Iliotibial band irritation caused by the EndoButton after anatomic double-bundle anterior cruciate ligament reconstruction: Report of two cases

• DOI: 10.1016/j.knee.2013.03.013
• 发表时间: 2013-08-01
• 期刊: KNEE
• 影响因子: 1.9
• 作者: Taketomi, Shuji;Inui, Hiroshi;Nakagawa, Takumi
• 通讯作者: Nakagawa, Takumi

Clinical outcome of anatomic double-bundle ACL reconstruction and 3D CT model-based validation of femoral socket aperture position.

解剖双束 ACL 重建和基于 3D CT 模型的股骨窝孔径位置验证的临床结果。

• DOI: 10.1007/s00167-013-2663-0
• 发表时间: 2013
• 期刊: Knee Surg Sports Traumatol Arthrosc.
• 作者: Taketomi S;Inui H;Nakamura K;Hirota J;Sanada T;Masuda H;Takeda H;Tanaka S;Nakagawa T.
• 通讯作者: Nakagawa T.

Transcriptional induction of ADAMTS5 by an NF-κB family member RelA/p65 in chondrocytes during osteoarthritis development

骨关节炎发展过程中软骨细胞中 NF-κB 家族成员 RelA/p65 转录诱导 ADAMTS5

• 发表时间: 2013
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Kobayashi H;Hirata M;Saito T;Itoh S;Chung UI;Kawaguchi H
• 通讯作者: Kawaguchi H

An Additional Reference Axis Improves Femoral Rotation Alignment in Image-Free Computer Navigation Assisted Total Knee Arthroplasty

• DOI: 10.1016/j.arth.2013.01.030
• 发表时间: 2013-05-01
• 期刊: JOURNAL OF ARTHROPLASTY
• 影响因子: 3.5
• 作者: Inui, Hiroshi;Taketomi, Shuji;Nakagawa, Takumi
• 通讯作者: Nakagawa, Takumi