Analysis of the TGF-b signaling in cartilage by nonlinear optics imaging combined with genetic engineering techniques

非线性光学成像结合基因工程技术分析软骨中TGF-b信号传导

• 批准号: 24659680
• 负责人: MIURA Hiromasa
• 金额: $ 2.33万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659680/
• 关键词: TGF-β; 軟骨; 軟骨変性; 変形性関節症; SHG; 2光子励起; Smurf; 二光子励起; 2光子励起顕微鏡

Multi-photon microscopy enables to characterize and evaluate alternation of collagen matrix in vivo and in vitro based on second harmonic generation (SHG) signal. We performed SHG imaging of the cartilage degeneration induced by enzyme treatment. In the result, changes in SHG signal intensity were observed in the experimental samples. We also established an animal osteoarthritis model by syndesmectomy using H2B-GFP line mice. We successfully evaluated over time the changes in cartilage matrix and chondrocytes by SHG and two-photon excited fluorescence. Furthermore, the articular cartilages of aged mice were observed as the same manner. In comparison with those of young mice, it was possible to evaluate them as spontaneous knee osteoarthritis. Our technique that combined genetic engineering and nonlinear interaction between the molecules and photons to approach the mechanism of cartilage degeneration is promising as a translational research leading to clinical application.

多光子显微镜能够基于二次谐波产生（SHG）信号在体内和体外表征和评估胶原基质的变化。我们对酶处理诱导的软骨退变进行了SHG成像。结果，在实验样品中观察到SHG信号强度的变化。我们还使用H2 B-GFP系小鼠通过韧带联合切除术建立了动物骨关节炎模型。我们通过SHG和双光子激发荧光成功地评估了软骨基质和软骨细胞随时间的变化。此外，以同样的方式观察老年小鼠的关节软骨。与年轻小鼠相比，可以将其评价为自发性膝关节骨关节炎。 我们的技术，结合基因工程和分子和光子之间的非线性相互作用，以接近软骨退变的机制是有前途的转化研究，导致临床应用。

项目成果

非線形光学技術を用いた変形性関節症の評価法の確立

利用非线性光学技术建立骨关节炎评估方法

• 发表时间: 2013
• 作者: 清松悠;大嶋佑介;疋田温彦;三浦裕正;今村健志
• 通讯作者: 今村健志

Evaluation of articular cartilage degeneration in mice based on second harmonic generation microscopy

基于二次谐波显微镜评价小鼠关节软骨退变

• 发表时间: 2013
• 作者: Hiroshi Kiyomatsu.,Yusuke Oshima.;Atsuhiko Hikita.;Hiromasa Miura.,Takeshi Imamura.
• 通讯作者: Hiromasa Miura.,Takeshi Imamura.

Evaluation of cartilage based on second harmonic generation microscopy

基于二次谐波发生显微镜的软骨评估

• 发表时间: 2014
• 作者: Hiroshi Kiyomatsu;Yusuke Oshima;Atsuhiko Hikita;Tadahiro Iimura;Takashi Saito;Tsuyoshi Miyazaki;Hiromasa Miura;Takeshi Imamura
• 通讯作者: Takeshi Imamura

Evaluation of osteoarthritis based on second harmonic generation microscopy

基于二次谐波显微镜的骨关节炎评估

• 发表时间: 2013
• 作者: Hiroshi Kiyomatsu;Yusuke Oshima;Atsuhiko Hikita;Hiromasa Miura;Takeshi Imamura
• 通讯作者: Takeshi Imamura

Evaluation of osteoarthritis based on second harmonic generation (SHG) imaging

基于二次谐波发生（SHG）成像的骨关节炎评估

• 发表时间: 2014
• 作者: 清松悠;大嶋佑介;疋田温彦;三浦裕正;今村健志
• 通讯作者: 今村健志