Analysis of commensal neisserial species as a genetic pool for drug resistant genes

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Various kinds of antimicrobial-resistant pathogens have emerged. In addition to pathogens causing nosocomial infections, pathogens for community-acquired infections also have developed their antimicrobial-resistance.n this project, we tried to understand the mode of emergence and acquisition of resistant gene. We analyzed total 107 commesal neisserial strains, which are speculated as a source of resistant genes for N. gonorrhoeae. We could detect ceftriaxone (CRO) resistant genes in the commesal strains. From the comparison of penA genes, newly identified penA from CRO-resistant commensal strains were similar (not identical) with penA (H041). Some unique amino acids observed in penA (H041) were shared with the CRO resistant strains. We designed real-time PCR assay system, which could detect penA (H041) specifically. We tried to detect CRO-resistant neisserial strains after CRO treatment for N. gonorrhoeae, but the resistant strains could not be emerged.