Establishment and functional analysis of induced pluripotent cancer cells in ovarian cancer

卵巢癌诱导多能癌细胞的建立及功能分析

• 批准号: 24659723
• 负责人: KURACHI Hirohisa
• 金额: $ 2.25万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659723/
• 关键词: 卵巣がん; 低酸素; microRNA; ハニカム膜; 細胞接着; 細胞増殖; 卵巣癌幹細胞; 卵巣明細胞腺癌; 卵巣癌

Honeycomb films (3D porous scaffold) exert an influence on cell adhesion and proliferation. Honeycomb films inhibited cell proliferation compared with control flat films in ovarian cancer cells, suggesting that honeycomb films have the possibility of inducing cancer cell to cancer stem cell and suppressing peritoneal spread of ovarian cancer. Ovarian cancer ascites were collected from patients who underwent surgery and we identified that primary CD11b+CD14+ cells, which were predominantly macrophages, are the major source of IL-6 production in an ovarian tumor microenvironment. When CD11b+CD14+ cells were co-cultured with cancer cells, the invasion and the proliferation of cancer cells were robustly promoted and these promotions were almost inhibited by the pretreatment of anti-IL-6R antibody (tocilizumab).

蜂窝膜（3D 多孔支架）对细胞粘附和增殖产生影响。与对照平板膜相比，蜂窝膜抑制了卵巢癌细胞的细胞增殖，表明蜂窝膜具有诱导癌细胞转变为癌症干细胞并抑制卵巢癌腹膜扩散的可能性。从接受手术的患者身上收集卵巢癌腹水，我们发现原代 CD11b+CD14+ 细胞（主要是巨噬细胞）是卵巢肿瘤微环境中 IL-6 产生的主要来源。当CD11b+CD14+细胞与癌细胞共培养时，癌细胞的侵袭和增殖被强烈促进，并且这些促进几乎被抗IL-6R抗体（托珠单抗）的预处理所抑制。

项目成果

Non-sex cord-stromal ovarian tumors frequently produce and secrete estrogen in postmenopausal women : impact on bone metabolism and abnormal endometrial histology

非性索间质卵巢肿瘤经常在绝经后妇女中产生和分泌雌激素：对骨代谢和异常子宫内膜组织学的影响

• DOI: 10.1210/jc.2013-1267
• 发表时间: 2013
• 期刊: J Clin Endocrinol Metab
• 作者: Matsumura S;Ohta T;Takahashi T;T Kurachi H
• 通讯作者: T Kurachi H

2年前の膀胱癌由来と思われる腹膜偽粘液腫の1例

2年前疑似膀胱癌引起的腹膜假粘液瘤一例

• 发表时间: 2013
• 作者: 小島原敬信;高橋俊文;松尾幸城;松村創平;小幡美由紀;松川淳;倉智博久
• 通讯作者: 倉智博久

Histopathological prognostic factors predicting para-aortic lymph node metastasis in patients with endometrioid uterine cancer

预测子宫内膜样子宫癌患者主动脉旁淋巴结转移的组织病理学预后因素

• DOI: 10.1016/j.ygyno.2010.05.004
• 发表时间: 2010
• 期刊: Gynecol Oncol
• 影响因子: 4.7
• 作者: Karube Y;Kurachi H
• 通讯作者: Kurachi H

化学療法後の卵巣癌再発巣におけるAktとERKの活性化は予後良好因子である

卵巢癌化疗后复发中 Akt 和 ERK 的激活是有利的预后因素

• 发表时间: 2010
• 作者: 清野学;太田剛;高橋俊文;逸見典子;須藤毅;本山悌一;倉智博久
• 通讯作者: 倉智博久

Identifying Prognostic Factors in Japanese Women with Pseudomyxoma Peritonei: A Retrospective Clinico-Pathological Study of the Tohoku Gynecologic Cancer Unit

• DOI: 10.1620/tjem.223.91
• 发表时间: 2011-02-01
• 期刊: TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
• 影响因子: 2.2
• 作者: Kojimahara, Takanobu;Nakahara, Kenji;Kurachi, Hirohisa
• 通讯作者: Kurachi, Hirohisa