Technical development of microscopic replantation of animal leg frozen with CAS freezer.

通常的冷冻过程会导致重要组织中的细胞完全死亡。细胞存活系统（Cells Alive System， CAS）是一种特殊的冷冻技术，在冷冻过程中将细胞死亡控制在最低限度。本研究的目的是发展动物断肢、CAS冷冻和解冻的再植技术。结果，虽然再植的腿最终导致完全坏死，但对未来取得了非常有用的发现。即CAS冷冻机性能的进步，使其能够更快、更均匀地冷冻，冷冻液的调整和制备将导致未来的成功。

Usual freezing process causes completely cell death in the vital tissue. Cells Alive System (CAS) is a particular freezing technique which restrains cell death during freezing at minimum. Purpose of this research was to develop replantation technique of amputated, CAS frozen, and thawed animal leg. As results, although replanted leg resulted in complete necrosis eventually, very useful findings for the future were achieved. That is, progress of performance of CAS freezer as to produce quicker and more uniform freezing, and adjustment and preparation of cryopreservation solution would lead to future success.