Development of biopolymer compound to elucidate the effect of glucan on innate immune system

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Several immune system cell-surface receptors are reported to be associated with osteoclastogenesis. Dectin-1, a lectin receptor for beta-glucan, is found predominantly on cells of the myeloid lineage. In the present study, we examined the effect of dectin-1 agonist, curdlan, on osteoclastogenesis. In dectin-1 over-expressing RAW 264.7 cells (d-RAW), curdlan suppressed receptor activator of nuclear factor-kappaB (NF-kappaB) ligand (RANKL)-induced osteoclast differentiation, bone resorption and actin-ring formation. Furthermore, curdlan inhibited RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1) expression.In conclusion, our results demonstrate that curdlan potentially inhibits osteoclast differentiation, especially NFATc1 expression. This suggests activation of curdlan-dectin-1 interaction critically regulates the genes required for osteoclastogenesis.