Function elucidation of the ALP in the pluripotent maintenance of pulpal stem cells and iPS cells and its application

我们检测了用于胚胎干细胞和iPS细胞多能性研究的碱性磷酸酶（ALP）的性质。牙髓干细胞（DPSC）不表现ALP活性，而诱导的iPS细胞表现出组织非特异性ALP活性。至于器官的来源，我们用于本研究的肿瘤细胞均表现出组织非特异性ALP的特征。这表明细胞通过成为肿瘤而失去了原ALP的类型，并开始表现出组织非特异性类型的性质。由于上述结果在细胞初始化与恶性转化和ALP之间的关联中很有趣，我们将继续研究。

We examined a property of the alkaline phosphatase (ALP) used for a pluripotent index in the study of embryonic stem cells and iPS cells. The dental pulp stem cells (DPSC) did not show ALP activity, but the induced iPS cells from DPSC showed the ALP activity of the tissue nonspecific type. As for the origin of the organ, the tumor cells which we used for this study showed all a characteristic of the tissue nonspecific type ALP. This suggests that the cells lost original type of the ALP by becoming a tumor, and came to show the nature of the tissue nonspecific type. As the above-mentioned results are interesting in an association between initialization of cells and malignant transformation and ALP, we continue the study.