Periodontal ligament cell growth with their stemness on reconstituted extracellular matrices mimicking stem cell niche

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Extracellular matrices formed by periodontal ligament cells (PDLs), mesenchymal stem cells(MSCs) and fibroblasts (NHDFs) were prepared as cell culture substrates by decellularization technique. Actin fibers and cell nuclei could not be observed after decellularization whereas they were clearly observed before decellularization, indicating that cellular components were removed from the substrates.PDLs can attach on these decellularized matrices. More PDLs attached on PDL-derived decellularized matrices than MSCs- and NHDFs-derived decellularized matrices. PDL can also grow on these decellularized matrices. However, the growth rates of PDLs on PDLs- and MSCs-derived decellularized matrices were lower than that on NHDF-derived decellularized matrices. It is expected that these decellularized matrices can be used as the substarates mimicking niche surrounding PDL in vivo.