The analysis of molecular mechanism of bone destruction using invitro cocultivation assay and application to a risk management in ameloblastoma

体外共培养分析骨质破坏的分子机制及其在成釉细胞瘤风险管理中的应用

• 批准号: 24659900
• 负责人: FUJII Tomomi
• 金额: $ 2.41万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659900/
• 关键词: 国際情報交換; インドネシア; エナメル上皮腫; 分子生物学

In this study, we immortalized cells which we obtained from the lesion of ameloblastoma and the oral mucosa of the healthy subject and established new ameloblastoma cell line (AM-3) and normal oral mucosa epithelium cell line (MOE-1). The expression of Wnt5a, Fz-2, MMP-2, -9 was higher remarkably in ameloblastoma cell line than normal oral mucosa epithelium cell line. Also, Wnt-3a treatment activated expression of MMP-9 of AM-3. Furthermore, osteoclasts differentiation was induced when we cocultivated with AM-3cells and RAW 264.7 cells which were osteoclastic precursors.

本研究从成釉细胞瘤病变和健康人口腔粘膜中分离细胞，建立成釉细胞瘤细胞系AM-3和正常口腔粘膜上皮细胞系莫伊-1。成釉细胞瘤细胞系中Wnt 5a、Fz-2、MMP-2、MMP-9的表达明显高于正常口腔黏膜上皮细胞系。此外，Wnt-3a处理激活AM-3的MMP-9的表达。此外，破骨细胞的前体细胞AM-3和RAW 264.7细胞共培养时，破骨细胞的分化被诱导。

项目成果

エナメル上皮腫由来細胞株の浸潤能におけるWntシグナルの意義

Wnt信号传导在成釉细胞瘤细胞系侵袭能力中的意义

• 发表时间: 2013
• 作者: 岐部俊郎;岸田昭世;比地岡浩志;宮脇昭彦;中村典史
• 通讯作者: 中村典史

A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis

• DOI: 10.1016/j.oooo.2013.03.005
• 发表时间: 2013-06-01
• 期刊: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
• 影响因子: 2.9
• 作者: Kibe, Toshiro;Fuchigami, Takao;Kishida, Shosei
• 通讯作者: Kishida, Shosei