Construction of fluorescence immunochromatography technique using a long persistent phosphor nanoparticles

免疫层析测定是一种简单快速的测试，它提供了一个结果，而不需要专门的和昂贵的设备。虽然已知荧光免疫层析测定与传统比色法相比对于检测样品具有高灵敏度，但是由于需要荧光检测器，因此缺乏使用的便利性。在此，我专注于一个长余辉陶瓷（LPC），它显示出强烈的长寿命余辉发光时，与紫外可见光照射，并解决了新的磷光免疫色谱技术的发展与长余辉陶瓷。采用球磨法制备了具有长余辉性能的纳米陶瓷。建立了在纳米粒子表面引入叶酸、抗体等生物分子的合成方法。

The immunochromatographic assay is a simple and rapid test, which provides a result without the need for specialized and costly equipment. Although it is known that a fluorescence immunochromatographic assay is high sensitive for detection of the sample as compared with a traditional colorimetric method, this is lacking in convenience of the use because of the necessary of a fluorescence detector. Herein, I focused on a long persistent ceramics (LPC), which shows intense long-lived afterglow luminescence when irradiated with UV-vis light, and addressed the development of new phosphorescence immunochromatographic techniques with the long persistent ceramics. Ball-milling was used to prepare the nanoparticles of ceramics with long persistent property. The synthetic method to introduce biomolecules such as folic acid and antibody on the surface of the nanoparticles has been established.