The role of Lefty1 expression cells during the ES cell self-organization.

Lefty1 表达细胞在 ES 细胞自组织过程中的作用。

• 批准号: 25650079
• 负责人: TAKAOKA Katsuyoshi
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25650079/
• 关键词: 再生医療; 自己組織化; ES細胞; 人工臓器; 位置情報; 未分化; 非対称性の起源

The acquirement of positional informations is essential for the making of structurally-complicated artificial organs. In mouse embryos, Lefty1 is a key player during the Left-right and anterior-posterior axis formation. And, it is known that Lefty1 is expressed is ES (Embryonic Stem) cells. In this study, I have tried to reveal the role of Lefty1 expression cells in the self-organization of ES cells.As a result, time-lapse observations of Lefty1 expressing cells in the formation of colony showed that the expression of Lefty1 started in edge cells of the ES colony.And, Lefty1,2 null ES cells normally formed colonies. Hereafter, I'm going to reveal which do Lefty1,2 null ES cells form embryoid bodies.

位置信息的获取对于结构复杂的人工器官的制造是必不可少的。在小鼠胚胎中，Lefty1是左右轴和前后轴形成过程中的关键角色。并且，已知Lefty1在ES（胚胎干）细胞中表达。本研究试图揭示Lefty 1表达细胞在ES细胞自组织中的作用，结果发现Lefty 1表达细胞在集落形成过程中的延时观察表明，Lefty 1的表达开始于ES集落的边缘细胞，Lefty 1，2缺失的ES细胞能正常形成集落。在下文中，我将揭示哪些Lefty1，2无效ES细胞形成胚状体。

项目成果

Origin of Anterior-Posterior axis formation in themouseembryo

小鼠胚胎前后轴形成的起源

• 发表时间: 2014
• 作者: Misako Okumura;Chisako Sakuma;Masayuki Miura and Takahiro Chihara;Katuyoshi Takaoka
• 通讯作者: Katuyoshi Takaoka

Establishment of Anterior-Posterior axis in the mouse embryo

小鼠胚胎前后轴的建立

• 发表时间: 2014
• 作者: Misako Okumura;Chisako Sakuma;Masayuki Miura and Takahiro Chihara;Katuyoshi Takaoka;Katsuyoshi Takaoka& Hiroshi Hamada;高岡勝吉;Katsuyoshi Takaoka& Hiroshi Hamada;Katsuyoshi Takaoka
• 通讯作者: Katsuyoshi Takaoka

マウス胚における非対称性の起源

小鼠胚胎不对称的起源

• 发表时间: 2014
• 作者: Misako Okumura;Chisako Sakuma;Masayuki Miura and Takahiro Chihara;Katuyoshi Takaoka;Katsuyoshi Takaoka& Hiroshi Hamada;高岡勝吉
• 通讯作者: 高岡勝吉

New Principles in Developmental Processes

• DOI: 10.1007/978-4-431-54634-4
• 发表时间: 2014
• 作者: H. Kondoh;A. Kuroiwa