Cell-loaded microgels as mechanical protection and controlled microenvironment for cells in bioinks (A06)

该项目的目的是产生尺寸均匀的负载细胞的微凝胶，并将其作为生物墨水的添加剂。微凝胶将在一侧充当在打印期间免受剪切力的机械保护，从而将生物织物窗口向具有更高粘度的生物油墨和向更高剪切力延伸。除此之外，生物化学功能化和调节微凝胶降解性将用于定制封装细胞的微环境并控制其行为。该项目旨在建立细胞负载微凝胶的悬浮液作为生物墨水，特别是用于同时打印不同类型的细胞。

Aim of this project is the generation of cell-loaded microgels with uniform size and their establishment as additives in bioinks. The microgels shall on the one side act as mechanical protection from shearforces during printing and thereby extend the biofabrication window towards bioinks with higher viscosity and towards higher shear forces. In addition to that, biochemical functionalization and adjusting microgel degradability shall be used to tailor the microenvironment of the encapsulated cells and control their behavior. Prospectively, this project aims at establishing suspensions of cell-loaded microgels as bioink especially for the simultaneous printing of different cell types.