Establishment of an animal model for Malignant hyperthermia

恶性高热动物模型的建立

基本信息

  • 批准号:
    01870067
  • 负责人:
  • 金额:
    $ 19.97万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
  • 财政年份:
    1989
  • 资助国家:
    日本
  • 起止时间:
    1989 至 1991
  • 项目状态:
    已结题

项目摘要

Malignant hyperthermia (MH) is one of the most hazardous complications encountered in anesthesia. It is assumed to be a genetic and metabolic disorder of the muscle, whose syndrome is triggered by anesthetics, depolarizing neuromuscular blocking agents and their related agents. The pure-line Pietrain pig is well known all over the world as a species that frequently develops porcine stress syndrome (PSS). PSS may be an animal model of MH observed in man. The purpose of this study was to import and culture the pure-line Pretrain pig and determine whether it is an animal model of human NM or not.The pure-line Pietrain pigs of Belgian were imported from the Netherlands. These pigs were bred and then classified whether positive for PSS (PSS+) or negative (PSS-) by the halothane inhalation test (halo-test). The rate of Ca-induced Ca release (CICR) of skeletal muscle was not significantly deferent between the two pigs, but their values were significantly higher than those of other strains of pigs (Landrace-Yorkshire). All the pigs were anesthetize with halothane or sevoflurane. MH syndrome was observed in Pietrain pigs which were either PSS+ or PSS-pigs following anesthesia with these inhalation anesthetics. PaCO2 was elevated at first and thereafter metabolic acidosis made progressed. Next, body temperature began to rise, followed by hyperkalemia, high serum CPK and GOT, and elevated of other enzyme values. At last, all the pigs died within 100 minutes during halothane inhalation without any therapy, but MH syndrome was not observed in the other strains pigs.We have concluded from these results that Pietrain pigs can be used as an animal model of human MH.
恶性高热(MH)是麻醉过程中最危险的并发症之一。它被认为是一种肌肉的遗传和代谢障碍,其综合征由麻醉剂、去极化神经肌肉阻滞剂及其相关药物触发。纯种皮特兰猪是全世界公认的易患猪应激综合征(PSS)的品种。PSS可能是在人类中观察到的MH的动物模型。本研究的目的是引进和培养纯系前期训练猪,并确定它是否为人类NM的动物模型。比利时纯系皮特兰猪从荷兰引进。用氟烷吸入试验(Halo-test)对这些猪进行PSS阳性(PSS+)或阴性(PSS-)分类。两种猪骨骼肌钙诱导释钙率(CICR)差异不显著,但显著高于其他品系猪(长白-约克夏)。所有猪均用氟烷或七氟醚麻醉。用这些吸入麻醉剂麻醉PSS+或PSS-猪后,皮特兰猪出现MH综合征。PaCO2先升高,后发展为代谢性酸中毒。其次,体温开始升高,其次是高钾血症,血清CPK和GOT升高,其他酶活性升高。最后,所有猪在吸入氟烷的过程中100分钟内死亡,而其他品系的猪均未观察到MH综合征。因此,皮特兰猪可以作为人MH的动物模型。

项目成果

期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
久保田 稔他: "悪性高熱症モデル豚(Piefrain pig)の麻酔経験" 第14回悪性高熱研究会. (1990)
Minoru Kubota等:“恶性高热模型猪(Piefrain猪)的麻醉经验”第14届恶性高热研究组(1990)。
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    0
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藤岡泰博他: "脱分極性筋弛緩薬サクシニルコリンクロライドとCaーusduced Ca release" 麻酔と蘇生. 25. (1989)
Yasuhiro Fujioka 等人:“去极化肌肉松弛剂氯化琥珀胆碱和 Ca 引起 Ca 释放”麻醉和复苏 25。(1989)
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    0
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海老 成直: "悪性高熱症モデルブタは畜産界ではどのように扱かわれているか" 医学のあゆみ. 158. 360 (1991)
Shigenao Ebi:“畜牧业如何治疗恶性高热模型猪?” 158. 360 (1991)
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  • 影响因子:
    0
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久保田 稔: "悪性高熱症モデル豚(pietlcin Pig)の麻酔経験" 麻酔と蘇生. 27(別). 87-95 (1991)
Minoru Kubota:“恶性高热模型猪(pietlcin 猪)的麻醉经验”麻醉和复苏 27(单独)。
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    0
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YUGE Osafumi其他文献

YUGE Osafumi的其他文献

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{{ truncateString('YUGE Osafumi', 18)}}的其他基金

Diagnosis of malignant hyperthermia susceptibility : by measuring change in myoplasmic calcium concentration in cultured human skeletal muscle cells and genetic analysis
恶性高热易感性诊断:通过测量培养的人体骨骼肌细胞中肌浆钙浓度的变化和遗传分析
  • 批准号:
    12470321
  • 财政年份:
    2000
  • 资助金额:
    $ 19.97万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
THE TRIAL STUDY FOR THE MINIMUM INVASIVE DIAGNOSTIC TEST FOR MHS -THE EFFECTS OF CAFFEINE AND HALOTHANE ON CULTURED HUMAN SKELETAL MUSCLE CELL MESURING INTRA CELLULAR Ca CONCENTRATION CONCENTRATION AND GENETIC ANALYSIS-
MHS微创诊断试验的试验研究-咖啡因和氟烷对培养的人体骨骼肌细胞测定细胞内Ca浓度的影响及遗传分析-
  • 批准号:
    08407052
  • 财政年份:
    1996
  • 资助金额:
    $ 19.97万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Effects of bolorile anesthetics in isolated myocordial beta-adrenergic receptor transmission
bolorile 麻醉剂对离体心肌 β-肾上腺素能受体传递的影响
  • 批准号:
    05671265
  • 财政年份:
    1993
  • 资助金额:
    $ 19.97万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Effects of hypoxia-reoxygenation on superoxide production and change of intra-cellular Ca^<++> of neutrophils
缺氧复氧对中性粒细胞超氧化物产生及细胞内Ca^<>变化的影响
  • 批准号:
    02670683
  • 财政年份:
    1990
  • 资助金额:
    $ 19.97万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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