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Microencapsulated Multicell Tumor Spheroids

微囊化多细胞肿瘤球体

基本信息

批准号：
01870085
负责人：
SASAKI Takehito
金额：
$ 8.96万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01870085/
关键词：
Radiotherapy Chemotherapy spheroid Microcapsule Radiosensitivity Solid tumor Radioresistance 細胞増殖

项目摘要

The purpose of this investitgation is to establish the assay system in vivo for evaluating the therapeutic effect of radiation or drugs on malignant solid tumors.1. Method of microcapsules FormationWe tried to set up the system for making microcapsules of perfect spherical shape using sodium alginate and poly-L-lysine. The size and shape of the microcapsules, membrane permeability depended on the degree of polymerization and sterilization method of the alginate, shape of air jet nozzle, flow rate of air and molecular weight of poly-L-lysine. It was concluded for obtaining microcapsules of about 500 mum diameter to use the sodium alginate of polymerization degree 118 and viscosity of 500 CP at 1% solution, the sterilization by filtration. The stability and permeability of the membrance was better to use poly-L-lysine of MW 22, 000 than that of lower or higher molecular weight.2. Proliferation of Cancer Cells and In Vivo Transplantation.Human cancer cells encapsulated in the microcapsules proliferated rapidly in situ to forny a multicell tumor spheroid in vitro. When these capsules were transplanted either intraperitoneally or subcutaneously, numerous blood cells were attached to the surface of the microcapsules and it became difficult to recover these microcapsules from mice with a longer time interval.3. Cell Survival Assay.A linear proportional relationship was obtained between the number of cells plated per well and the mean optical density per well. The slope of the curve depended upon the surviving fraction of cells. Using this relationship. ' the survival curves of GBA-7 and TE-2 human cancer cells against the dose of X-ray were obtained. The result indicates that GBA-7 cells were more resistant to X-ray than TE-2 cells which is comparable to that obtained from the colony forming assay.

本研究的目的是建立评价放射线或药物对恶性实体瘤治疗效果的体内测定系统。 1．微胶囊的形成方法我们尝试建立使用海藻酸钠和聚-L-赖氨酸制造完美球形微胶囊的系统。微胶囊的尺寸和形状、膜的渗透性取决于海藻酸盐的聚合度和灭菌方法、空气喷嘴的形状、空气的流速和聚-L-赖氨酸的分子量。结论是采用聚合度118、粘度500CP的海藻酸钠1％溶液，过滤除菌，即可得到直径约500μm的微胶囊。使用MW 22, 000的聚L-赖氨酸膜的稳定性和渗透性优于较低或较高分子量的膜。 2．癌细胞增殖和体内移植。封装在微胶囊中的人类癌细胞在体外迅速原位增殖，形成多细胞肿瘤球体。当这些胶囊被移植到腹腔或皮下时，大量的血细胞附着在微胶囊的表面，并且在较长的时间间隔内从小鼠中回收这些微胶囊变得困难。 3．细胞存活测定。每孔铺板的细胞数与每孔平均光密度之间获得线性比例关系。曲线的斜率取决于细胞的存活分数。利用这种关系。获得GBA-7和TE-2人类癌细胞相对于X射线剂量的存活曲线。结果表明，GBA-7 细胞比 TE-2 细胞对 X 射线具有更强的抵抗力，这与集落形成测定中获得的结果相当。