Microencapsulated Multicell Tumor Spheroids

本研究的目的是建立一种评价放射或药物对恶性实体瘤治疗效果的体内检测系统。微胶囊的制备方法我们尝试用海藻酸钠和聚l -赖氨酸制备球形微胶囊。微胶囊的大小和形状、膜的通透性取决于海藻酸盐的聚合程度和灭菌方式、空气喷嘴的形状、空气流速和聚l -赖氨酸的分子量。结果表明，在1%溶液中，聚合度为118、粘度为500 CP的海藻酸钠可获得直径约500 μ m的微胶囊，并经过滤灭菌。分子量为22,000的聚l -赖氨酸膜的稳定性和通透性均优于分子量较小或较大的聚l -赖氨酸膜。癌细胞的增殖和体内移植。包被微胶囊的人癌细胞在体外快速原位增殖，形成多细胞肿瘤球体。当这些胶囊被腹腔或皮下移植时，大量的血细胞附着在微胶囊的表面，并且这些微胶囊很难从小鼠身上恢复，时间间隔较长。细胞存活试验。在每孔镀的细胞数和每孔的平均光密度之间得到了线性比例关系。曲线的斜率取决于细胞的存活比例。利用这种关系。获得GBA-7和TE-2人癌细胞对x射线剂量的生存曲线。结果表明，GBA-7细胞比TE-2细胞对x射线的抗性更强，这与集落形成试验的结果相当。

The purpose of this investitgation is to establish the assay system in vivo for evaluating the therapeutic effect of radiation or drugs on malignant solid tumors.1. Method of microcapsules FormationWe tried to set up the system for making microcapsules of perfect spherical shape using sodium alginate and poly-L-lysine. The size and shape of the microcapsules, membrane permeability depended on the degree of polymerization and sterilization method of the alginate, shape of air jet nozzle, flow rate of air and molecular weight of poly-L-lysine. It was concluded for obtaining microcapsules of about 500 mum diameter to use the sodium alginate of polymerization degree 118 and viscosity of 500 CP at 1% solution, the sterilization by filtration. The stability and permeability of the membrance was better to use poly-L-lysine of MW 22, 000 than that of lower or higher molecular weight.2. Proliferation of Cancer Cells and In Vivo Transplantation.Human cancer cells encapsulated in the microcapsules proliferated rapidly in situ to forny a multicell tumor spheroid in vitro. When these capsules were transplanted either intraperitoneally or subcutaneously, numerous blood cells were attached to the surface of the microcapsules and it became difficult to recover these microcapsules from mice with a longer time interval.3. Cell Survival Assay.A linear proportional relationship was obtained between the number of cells plated per well and the mean optical density per well. The slope of the curve depended upon the surviving fraction of cells. Using this relationship. ' the survival curves of GBA-7 and TE-2 human cancer cells against the dose of X-ray were obtained. The result indicates that GBA-7 cells were more resistant to X-ray than TE-2 cells which is comparable to that obtained from the colony forming assay.