Chemo-enzymatic Manipulation of RNA: Sequence-specifity via Cooperative Effects (B18*)

RNA 的化学酶操作：通过协同效应实现序列特异性 (B18*)

• 批准号: 398186078
• 金额: --
• 依托单位: Department Chemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/398186078?language=en
• 关键词: Chemo; enzymatic; Manipulation; RNA; Sequence

Sequence-specific manipulation of mRNA provides access to studying and controlling gene expression at the post-transcriptional level which is of particular importance for molecular biology and cellular applications. The 5′ cap is present in all eukaryotic mRNAs and m6A is the most abundant internal modification in mRNA. Both modifications control numerous interactions but currently cannot be installed or removed in a sequence-specific manner. This project aims to obtain sequence-specific mRNA manipulation at these modifications by creating fusion constructs of RNA-modifying enzymes with RNA-binding proteins or RNA-protein complexes. The precise manipulation of modifications will be used to label or isolate a specific transcript and to evaluate biological effects, particularly translation efficiency and stability.

mRNA的序列特异性操作提供了在转录后水平研究和控制基因表达的途径，这对于分子生物学和细胞应用特别重要。5′帽存在于所有真核生物mRNA中，m6 A是mRNA中最丰富的内部修饰。这两种修改都控制着大量的交互，但目前无法以特定于序列的方式安装或删除。该项目旨在通过创建RNA修饰酶与RNA结合蛋白或RNA-蛋白复合物的融合构建体，在这些修饰处获得序列特异性mRNA操纵。修饰的精确操作将用于标记或分离特异性转录物并评估生物学效应，特别是翻译效率和稳定性。