Challenge to microbial synthesis of block copolyesters by regulated gene expression

本研究旨在通过调控生物合成基因的表达来合成两种单体高度嵌段共价结合的聚羟基烷酸酯（PHA）共聚物。首先，在大肠杆菌中建立了适于调控基因表达的共聚物生物合成途径。然后，重组E.构建了具有调控PHA生物合成途径的大肠杆菌，目前正在进行菌株的PHA生物合成和积累PHA的结构分析。

This study aimed at microbial synthesis of polyhydroxyalkanoate (PHA) copolymers in which two kinds of monomers were highly block-covalently bonded by regulated expression of the biosynthetic genes. Firstly, copolymer biosynthesis pathways suitable for regulatory gene expression were established in Escherichia coli. Then, recombinant strains of E. coli equipped with a regulatory PHA biosynthesis pathways were constructed, and PHA biosynthesis by the strains and structural analyses of accumulated PHAs are nowunder progress.