Effects of intestinal bacterial adhesion to fibers on intestinal microflora

经纤维素柱纯化的乳酸乳球菌IL 1403细胞表面蛋白经鉴定为DnaK、GroEL、GAPDH等，在pH7.0时，荧光标记的DnaK对纤维素、粘蛋白、几丁质和IL 1403细胞的吸附常数分别为（2.7±0.9）×10^6、（2.5±0.5）×10^5、（3.7±2.2）× 10^5、（8.7±3.2）× 10^5M ^<-1>。在pH4.0时，纤维素和粘蛋白的吸附常数分别为（5.7 ± 0.2）× 10 ~ 6和（4.4 ± 1.0）× 10 ~ 6 M ^（-1）。这些结果意味着乳酸菌在产生乳酸时更紧密地粘附在肠表面。

Surface proteins from Lactococcus lactis IL1403 cells purified by cellulose column were identified to be DnaK, GroEL, GAPDH and so on. The adsorption constants of the fluorescence labeled DnaK at pH 7.0 were estimated to be(2.7±0.9)×10^6、(2.5±0.5)×10^5、(3.7±2.2)×10^5、(8.7±3.2)×10^5 M^<-1> for cellulose, mucin, chitin and IL1403 cells, respectively. At pH 4.0, the adsorption constants for cellulose and mucin were estimated to be(5.7+-0.2) 10^6 and(4.4+-1.0) 10^6M^(-1), respectively. These results mean that lactic acid bacteria adhere to intestinal surface more tightly when they produce lactic acid.