Analysis of epigenetic alternation in diabetic model cells

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The diabetic model cells were constructed by using a cell-resealing technique, which enables the exchange of cytosol in the cells. We observed that GFP-conjugated MeCP2, a methylated DNA binding protein, diffused throughout cytosol in diabetic model cells, which suggested the possibility that the epigenetics could be modified in diabetic model cells. In addition, microarray analysis was carried out to find the genes, which were up- or down-regulated in diabetic model cells, and revealed that expression of several genes were changed only by the introduction of diabetic liver cytosol into the cells. Although DNA methylation of the candidate genes is being examined now, this system could be a prototype for extracting the candidate genes, which could show the epigenetic alternation in a correlation with diabetic condition.