Revolution of Phylogenetic Analysis with Next Generation Sequencers

这项研究的目的是对在系统发育中占有关键地位的生物的基因组和转录组进行测序，以便能够以很高的统计置信度解决诸如深分支等困难的系统发育问题。对估计基因组大小为386 Mb的地钱植物Jungermannia infusca进行了测序和组装，表明对小于400 Mb的核基因组进行测序实际上是可行的，并且当人们想要获得低表达水平的基因和非常长的转录本时，可能比聚焦RNA-Seq便宜。

This research aimed at sequencing both the genome and the transcriptome of organisms that occupy pivotal position in the phylogeny to allow resolution of difficult phylogenetic problems such as deep branches with high statistical confidence. A liverwort species, Jungermannia infusca, with an estimated genome size of 386 Mb was sequenced and assembled, indicating that sequencing nuclear genome of less than 400 Mb is realistically doable, and could be less expensive than focusing RNA-seq when one want to obtain low-expression level genes and very long transcripts.