Revolution of Phylogenetic Analysis with Next Generation Sequencers

下一代测序仪的系统发育分析革命

• 批准号: 23657063
• 负责人: NISHIYAMA Tomoaki
• 金额: $ 2.41万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657063/
• 关键词: ゲノム; トランスクリプトーム; 系統解析; 全ゲノム解析; オルガネラ; RNA-seq

This research aimed at sequencing both the genome and the transcriptome of organisms that occupy pivotal position in the phylogeny to allow resolution of difficult phylogenetic problems such as deep branches with high statistical confidence. A liverwort species, Jungermannia infusca, with an estimated genome size of 386 Mb was sequenced and assembled, indicating that sequencing nuclear genome of less than 400 Mb is realistically doable, and could be less expensive than focusing RNA-seq when one want to obtain low-expression level genes and very long transcripts.

本研究旨在对在系统发育中占据关键地位的生物的基因组和转录组进行测序，以解决困难的系统发育问题，例如具有高统计置信度的深分支。对估计基因组大小为386 Mb的苔类物种Jungermannia infusca进行测序和组装，表明对小于400 Mb的核基因组进行测序是现实可行的，并且当人们想要获得低表达水平基因和非常长的转录物时，可能比聚焦RNA-seq更便宜。