Ability to form meiotic spindle is dependent on GV material

在产卵前，存在于GV附近的母紫菀在GVBD期间受到GV可溶性成分的影响，并获得形成功能纺锤体的能力。然后，产生的卵母细胞在MI处受精。尽管精子中心体形成精子母细胞，但它们要等到卵母细胞减数分裂完成后才参与细胞周期事件：在卵母细胞减数分裂时，它们不能形成功能纺锤体，因为GV成分已经分散在细胞质中。如果在GVBD或MI之前将卵母细胞从卵巢中分离出来进行人工授精，那么在GVBD期间，动物极点的精子会受到GV成分的影响，形成异常的多极纺锤体，在MI和MI期间与母体染色体相互作用。因此，MI的产卵和受精对于保护成熟的卵母细胞免受父亲中心体异常多极纺锤体的形成非常重要。

Before spawning, maternal asters existing near GV are affected by soluble components of GV other than chromosomes during GVBD, and acquire the ability to form functional spindles. Then, spawned oocytes are fertilized at MI. Although centrosomes of sperm form sperm asters, they wait to participate in cell cycle events until oocyte meiosis is completed: They cannot form functional spindles during meiotic division of oocytes, because GV components have been dispersed throughout the cytoplasm. When oocytes are isolated from the ovaries and artificially inseminated before GVBD or MI, polyspermy occurs and sperm asters at the animal pole are affected by GV components during GVBD and form abnormal multi-polar spindles interacting with maternal chromosomes during MI and MII. Thus, both spawning and fertilization at MI are important for maturing oocytes to be protected from formation of abnormal multi-polar spindles by paternal centrosomes.