Genetic background of tradeoffs related to the adaptation to different light conditions: molecular evolutionary ecology using two Viola

与适应不同光照条件相关的权衡的遗传背景：使用两种中提琴的分子进化生态学

• 批准号: 23657158
• 负责人: TOYAMA Hironori
• 金额: $ 2.33万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657158/
• 关键词: 雑種集団; ゲノムの置き換わり; AFLP; QTL; 光環境

We focused hybrid area of two closely related Viola species that was formed by invasions of V. eizanensis preferring more shaded places to V. chaerophylloides preferring more open places. 339 AFLP loci were used to estimate genetic architecture and generation of hybrids. Maternal inheritance was confirmed thorough sequencing of cpDNA atpI-atpH. Phenotypic variations were measured by leaf thickness and leaf dissection that were diversified between species. Molecular analysis showed that a genome of hybrid individuals was replaced by V. eizanensis. Significant correlations were observed between hybrid indices and two leaf traits. These results suggested adaptive genes of V. eizanensis to shaded habitats were fixed among hybrids and two leaf traits were important for their adaptation to light environments. For QTL analysis, we measured 5 traits for 443 F2 individuals and analyzed AFLP patterns.

我们重点研究了两个紧密相关的堇菜属物种的杂交区域，这是由喜欢更阴凉的地方的V. eizanensis比喜欢更开阔的地方的V. chaerophylloides入侵形成的。利用339个AFLP位点对杂种后代的遗传结构和世代进行了分析。通过cpDNA atpI-atpH测序证实了母系遗传。表型变异是通过不同物种之间的叶厚度和叶解剖来测量的。分子生物学分析表明，杂种个体的基因组被V. eizanensis所取代。杂种指数与两个叶片性状间存在显著相关。这些结果表明，高山葡萄对遮荫生境的适应基因在杂种中是固定的，两个叶片性状对高山葡萄对光环境的适应起着重要作用。在QTL分析中，我们对443个F2个体的5个性状进行了检测，并分析了AFLP图谱。

项目成果

Hironori Toyama

富山弘则

• 发表时间: 2011
• 作者: Hironori Toyama;Tetsukazu Yahara;Identification using DNA barcoding of forest trees in Cambodia.
• 通讯作者: Identification using DNA barcoding of forest trees in Cambodia.

森林動態による系統的群集構造の変化-カンボジアの森林プロットを用いて-

由于森林动态而导致的系统群落结构的变化 - 使用柬埔寨的森林地块 -

• 发表时间: 2013
• 作者: Hironori Toyama;Shuichiro Tagane;Phourin Chhang;Tsuyoshi Kajisa;Ryuji Ichihashi;Vanna Samreth;Vuthy Ma;Heng Sokh;Ayumi Katayama;Hiroki Itadani;Makiko Tateishi;Yuya Tachiki;Keiko Mase;Yusuke Onoda;Nobuya Mizoue;Hidenori Tachida and Tetsukazu;H. Toyama and T. Yahara;遠山弘法;遠山弘法;Toyama H;遠山弘法
• 通讯作者: 遠山弘法

Evaluation of biodiversity in Cambodia. I. Identification using DNA barcoding of forest trees in Cambodia. II. Preliminary results of phylogenetic community structure

柬埔寨生物多样性评估。

• 发表时间: 2011
• 作者: Toyama;H
• 通讯作者: H

Inventory of the Woody Flora in Permanent Plots of Kampong Thom and Kampong Chhnang Provinces

磅同省和磅清扬省永久地块木本植物区系清查

• 发表时间: 2013
• 期刊: Cambodia. Acta Phytotax. Geobot
• 作者: Toyama;H.;S. Tagane;P. Chhang;T. Kajisa;R. Ichihashi;V. Samreth;V. Ma;H. Sokh;A. Katayama;H. Itadani;M. Tateishi;Y. Tachiki;K. Mase;Y. Onoda;N. Mizoue;H. Tachida & T. Yahara
• 通讯作者: H. Tachida & T. Yahara

Forest plot studies using DNA barcoding

使用 DNA 条形码进行森林图研究

• 发表时间: 2012
• 作者: Toyama;H.;S. Tagane;R. Ichihashi;Y. Onoda
• 通讯作者: Y. Onoda