How do non-signalsequence small peptides transmit intercellular signals?

非信号序列小肽如何传递细胞间信号？

• 批准号: 23657152
• 负责人: KAGEYAMA Yuji
• 金额: $ 2.5万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657152/
• 关键词: ショウジョウバエ; 短鎖ペプチ; 細胞間シグナル伝達; 発生遺伝; 短鎖ペプチド; シグナル伝達; マイクロペプチド; 細胞間情報伝達; 細胞突起形成

The polished ricegene in Drosophilaencodes extremely small 11 or 32-aa long peptides (PRI peptides) that do not include any signal sequences, although genetic analysis have shown that prishows non-cell autonomous effects to neighboring cells. To elucidate molecular mechanisms underlying the non-cell autonomous signaling by the prigene, we tried to identify PRI peptides-associating proteins using cultured cells. Although we failed to raise specific antibody against PRI peptides, fusion peptides with molecular tags were effectively precipitated with anti-tag antibodies. We identified several proteins that are co-precipitated with PRI fusion peptides.

果蝇的抛光米基因编码非常小的11或32-aa长的肽（PRI肽），不包括任何信号序列，尽管遗传分析表明pris对邻近细胞具有非细胞自主作用。为了阐明prigene非细胞自主信号传导的分子机制，我们试图用培养细胞鉴定PRI肽相关蛋白。虽然我们未能获得针对PRI肽的特异性抗体，但具有分子标签的融合肽可以有效地与抗标签抗体沉淀。我们鉴定了几种与PRI融合肽共沉淀的蛋白质。

项目成果

Physiological roles of MRE32 RNA in Drosophila optic lobe and wing development.

MRE32 RNA 在果蝇视叶和翅膀发育中的生理作用。

• 发表时间: 2012
• 作者: Sachi Inagaki;Masanao Sato;Yasuo Fukami;Yuji Kageyama
• 通讯作者: Yuji Kageyama

Physiological roles of MRE32 RNA in Drosophilaoptic lobe and wing development

MRE32 RNA 在果蝇视叶和翅膀发育中的生理作用

• 发表时间: 2012
• 作者: Sachi Inagaki;Masanao Sato;Yasuo Fukami,Yuji Kageyama
• 通讯作者: Yasuo Fukami,Yuji Kageyama

A small peptide gene polished riceis essential for trichome formation and metamorphosis in Drosophila

精米小肽基因对于果蝇毛状体形成和变态至关重要

• 发表时间: 2011
• 作者: Yoshiko Hashimoto;Kaori Niimi;Takefumi Kondo;Yuji Kageyama
• 通讯作者: Yuji Kageyama

The Binding of Multiple Nuclear Receptors to a Single Regulatory Region Is Important for the Proper Expression of EDG84A in Drosophila melanogaster

• DOI: 10.1016/j.jmb.2012.10.020
• 发表时间: 2013-01-09
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Akagi, Kazutaka;Kageyama, Yuji;Ueda, Hitoshi
• 通讯作者: Ueda, Hitoshi