Chemical biology for establishment of high efficient gene targeting system

为了在高等植物中建立高效的同源重组（HR）基因靶向系统，筛选了能够提高同源重组频率的化学物质。在237种化学物质中，利用模型系统估算拟南芥植物的HR频率，成功筛选出15种能提高HR频率的化学物质。DNA双链断裂（DSBs）诱导的Rad51基因的表达分析表明，3种化学物质可以在不进一步诱导DSBs的情况下提高HR频率。

For the establishment of a high efficient gene targeting system via homologous recombination (HR) in higher plants, chemicals that can increase HR frequency were screened. Among 237 kinds of chemicals, 15 chemicals that can increase HR frequency were successfully selected using model system to estimate HR frequency in Arabidopsis plants. The expression analysis of Rad51 gene, which is reported to be induced by DNA double strand breaks (DSBs) showed that 3 chemicals can enhance HR frequency without further inducing DSBs.