Molecular ecological analyses of nitrogen-fixing bacterial consortia in rice rhizospere and plant

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Relationship between nitrogen-fixing activity of Klebsiella oxytoca R16, a diazotroph living in rice rhizosphere, and its bacterial interaction in rhizosphere flora was analyzed. Strain R16 was cultivated together with 9 strains of non-diazotroph isolated form paddy soil. Pseudomonas sp. RKF06 enhanced the nitrogen-fixing activity of R16 significantly. In inoculation assay of R16 into the rhizosphere of rice, R16 showed higher nitrogen-fixing activity when strain RKF06 was inoculated simultaneously. This finding will be a new manner to take advantage of diazotrophs.