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Studies on the determination of blood groups from small amounts of bloodstains by use of enzyme-linked immunosorbent assay(ELISA).

酶联免疫吸附试验（ELISA）测定少量血迹血型的研究。

基本信息

批准号：
60870026
负责人：
TAKATORI Takehiko
金额：
$ 3.2万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870026/
关键词：
ELISA ELFA ABC Bloodstain Saliva Nail Lewis blood group 自動化アビジン・ビオチン複合体

项目摘要

Enzyme-linked immunosorbent assay(ELISA) or enzyme-linked fluoroimmunosorbent assay(ELFA) with an avidin-biotin complex(ABC) was applied to the determination of ABH or Lewis blood group from salivas, Bloodstains and nails, and the results were as follows.1. ABH group of small amounts of non-secretion salivas was typed precisely by the present ELISA, although that of those was not determined by a conventional absorption inhibition test.2. The Lewis typing of bloodstains could be determined by the ELISA using monoclonal anti-Lea and anti-leb antibodies. About 1 mg of the stains was enough to type each Lewis substance reliably.3. The ELFA procedure was established. About 0.1 mg of the bloodstains was enough to type each Lewis antigen by this method.4. The Lewis typing of Salivastains was done by the ELISA. Although Lea substance in Le(a-b+)type saliva was secreted in various ratios, the level of Leb substance was superior to that of Lea antigen. Likewise, the same results in Le(a+b-)type salivastains were obtained. The level of both Lea Leb substances in Le(a-b-)type salivastains was always low compared to that of the other two types. The Lewis typing, therefore, was determined easily by measuring 2 to 3 points of serial two-fold saliva dilutions.5. It was evident taht nails contain the Lewis substances. However, the level of the Lewis substances did not reflex that of the corresponding erythrocytes.6. MN blood typing in bloodstains was also tested by the ELISA, but satisfactory results were not obtained. This may be attributed to the antibodies used.7. A software was established to automate the determination of blood grouping; it took only 3 to 4 min to read the data precisely by using this software.

采用酶联免疫吸附试验（ELISA）或酶联免疫荧光法（ELFA）检测唾液、血痕和指甲中ABH或刘易斯血型，结果如下. ABH组中少量无分泌唾液的ELISA分型准确，而常规的吸收抑制试验不能确定.用抗Lea和抗Leb单克隆抗体建立的ELISA可用于血痕的刘易斯分型。大约1毫克的染色剂足以可靠地分型每种刘易斯物质。建立了ELFA程序。每种刘易斯抗原的分型量约为0.1mg.用酶联免疫吸附试验（ELISA）进行唾液斑的刘易斯分型。虽然Le（a-b+）型唾液中莱亚物质的分泌比例不同，但Leb物质的分泌水平上级莱亚抗原。同样，在Le（a+B-）型唾液抑制剂中获得了相同的结果。Le（a-b-）型唾液分泌抑制剂中的莱亚Leb物质水平始终低于其他两种类型。因此，通过测量连续两倍唾液稀释液的2到3个点，可以很容易地确定刘易斯分型。很明显指甲里含有刘易斯物质。但刘易斯物质的水平并不能反映相应红细胞的水平。6.用酶联免疫吸附试验（ELISA）对血痕进行了MN血型分型，但未获得满意结果。这可能是由于使用的抗体。7.建立了一套血型鉴定自动化软件，使用该软件仅需3 ~ 4 min即可准确读取数据。