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Application of models of spasticity and spastic paralysis

痉挛和痉挛性麻痹模型的应用

基本信息

批准号：
60870092
负责人：
FUKUDA Hideomi
金额：
$ 3.52万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870092/
关键词：
Decerebrate rigidity Chlorpromazine Mephenesin Spastic paralysis 痙縮固縮バクロフェン

项目摘要

Intercollicular decerebrate rigidity (so-called <gamma> -rigidity) and anaemic decerebrate rigidity ( <alpha> -rigidity) have been used in pharmacological and physiological studies in cats. Because rats are usually used in behavioural and neurochemical studies for their ease of handling, however, we have produced both types of rigidity using rats in the evalustion of centrally acting muscle relaxants (Fukuda et al., 1974; Togari et al., 1978).The rate of success of intercollicular decerebrate rigidity has not been high probably due to compression of the brain by bleeding or the difficulty involved in precisely controlling the spatula. In order to avoid such problems, radio frequency (RF) lesion of the midbrain was produced in attempts to obtain a good preparation of rigidity and to establish an easy method of decerebration in rats.The effects of chlorpromazine (CPZ), a frug which preferentially depresses intercollicular decerebrate rigidity, and mephenesin, a centrally acting muscle relaxant, were evaluated on the RF decerebrate rigidity.1. Bilateral RF lesions of the midbrain were produced in an attempt to establish a non-bleeding method of producing decerebrate rigidity in rats.2. Marked extensor rigidity occurred with a high reproducibillity in hindlimbs without the appearance of voluntary movement. Stretch reflex tension induced by repetitive dorsiflexion of the hindfeet was employed as a measure of the intensity of rigidity.3. CPZ-HCL (0.1-1 mg/kg, i.v.) and mephenesin (10-50 mg/kg, i.v.) which depress intercollicular decerebrate rigidity, reduced the RF decerebrate rigidity.4. These results suggest that RF decerebrate rigidity can be used instead of intercollicular transection rigidity in the pharmacological and physiological studies.

在猫的药理学和生理学研究中，已经使用了collcolle decereate刚性（所谓的<gamma> -刚性）和贫血性decereate刚性（<alpha> -刚性）。然而，由于在行为和神经化学研究中通常使用大鼠，因为它们易于操作，因此我们在评估中枢作用的肌肉松弛剂时使用大鼠产生了两种类型的僵硬（Fukuda et al., 1974; Togari et al., 1978）。肩胛间去脑性僵硬的成功率并不高，可能是由于出血压迫大脑或难以精确控制刮刀。为了避免这些问题，我们制作了中脑射频损伤，试图获得良好的刚性准备，并建立一种简单的大鼠去脑方法。研究了氯丙嗪（CPZ）和甲非那辛（mephenesin）两种药物对大鼠肩关节间失觉刚度的影响。我们制作了双侧中脑射频病变，试图建立一种不出血的方法来产生大鼠的失智性僵硬。明显的伸肌强直发生，后肢可重复性高，但无自主运动的表现。由后脚重复性背屈引起的拉伸反射张力被用作测量刚性强度的指标。CPZ-HCL （0.1-1 mg/kg，静脉注射）和mephenesin （10-50 mg/kg，静脉注射）降低了颈间失觉刚度，降低了RF失觉刚度。这些结果表明，在药理学和生理学研究中，可以使用RF去椎体刚度来代替颈间横断刚度。