Development of Directly Coupled Supercritical Fluid Chromatograph-Mass Spectrometer System

超临界流体色谱（SFC）作为一种快速、有效的分离非挥发性和热不稳定化合物的技术得到了广泛的研究。由于SFC的大多数流动相，如二氧化碳和戊烷的挥发性比普通HPLC高得多，因此SEC与质谱仪（SFC- ms）直接耦合系统基本上是可行的。在本项目中，通过最初为HPLC-MS开发的真空雾化接口，将带有熔融二氧化硅填充毛细管柱的SFC与四极柱质谱直接耦合。因此，所开发的SFC-MS系统成功地应用于各种非挥发性样品，如聚苯乙烯低聚物、非离子表面剂，它们是由聚合物、邻苯二甲酸酯、甘油三酯、聚乙二醇（PEG）衍生物和维生素组成的复杂混合物。

Supercritical fluid chromatography(SFC) has been vigorously investigated and recognized as a rapid and powerful technique for separating non-volatile and thermally unstable compounds. Sine most of the mobile phases for SFC such as carbon dioxide and pentane have much higher volatility than those for ordinary HPLC, SEC is principally much feasible to make a direct coupling system with a mass spectrometr(SFC-MS). in this project, the SFC with the fused silica packed capillary column was directly coupled with a quadrupole MS through a vacuum nebulizing interface originally developed for HPLC-MS. Thus developed SFC-MS system was successfully applied to various non-volatile samples such as polystyrene oligomers, non-ionic surface agentswwhich are composed of complexed mixture of of origomeric polymers, phthalates, triglycerides, polyethylene glycol(PEG)derivatives and vitamins.