Development of Directly Coupled Supercritical Fluid Chromatograph-Mass Spectrometer System

直接耦合超临界流体色谱-质谱联用系统的研制

• 批准号: 61850139
• 负责人: TSUGE Shin
• 金额: $ 10.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61850139/
• 关键词: Supercritical fluid chromatography; Supercritical fluid chromatography-mass spectromtry System; Vitamin analysis; Oligomeric polymer analysis; Non-ionic surface agents; トリグリセリド分析; 超臨界流体クロマトグラフィーー質量分析法直結システム; ポリエチレングリコール; エポキシプレポリマー; オリゴマー; 超臨界液体

Supercritical fluid chromatography(SFC) has been vigorously investigated and recognized as a rapid and powerful technique for separating non-volatile and thermally unstable compounds. Sine most of the mobile phases for SFC such as carbon dioxide and pentane have much higher volatility than those for ordinary HPLC, SEC is principally much feasible to make a direct coupling system with a mass spectrometr(SFC-MS). in this project, the SFC with the fused silica packed capillary column was directly coupled with a quadrupole MS through a vacuum nebulizing interface originally developed for HPLC-MS. Thus developed SFC-MS system was successfully applied to various non-volatile samples such as polystyrene oligomers, non-ionic surface agentswwhich are composed of complexed mixture of of origomeric polymers, phthalates, triglycerides, polyethylene glycol(PEG)derivatives and vitamins.

超临界流体色谱（SFC）已被广泛研究并被认为是一种快速而强大的分离非挥发性和热不稳定化合物的技术。由于 SFC 的大多数流动相（例如二氧化碳和戊烷）的挥发性比普通 HPLC 的流动相高得多，因此 SEC 原则上与质谱仪（SFC-MS）建立直接耦合系统是非常可行的。在该项目中，配备熔融石英填充毛细管柱的 SFC 通过最初为 HPLC-MS 开发的真空雾化接口直接与四极杆 MS 耦合。由此开发的SFC-MS系统成功应用于各种非挥发性样品，如聚苯乙烯低聚物、由低聚聚合物、邻苯二甲酸酯、甘油三酯、聚乙二醇(PEG)衍生物和维生素的复合混合物组成的非离子表面剂。

项目成果

Kozo, MATSUMOTO: "Development of Directly Coupled Supercritical Fluid Chromatography-Mass Spectrometry with Self-Spouting and Vacuum Nebulizing Interface" Analytical Sciences. 2. 3-7 (1986)

Kozo, MATSUMOTO：“具有自喷流和真空雾化接口的直接耦合超临界流体色谱-质谱法的开发”分析科学。

Kozo, MATSUMOTO: "Application of HPLC/MS with Self-spouting and Vacuum Nebulizing Assisted Interface to the Analysis of Triglycerides" Mass Spectroscopy. 34. 34-40 (1986)

Kozo, MATSUMOTO：“具有自喷流和真空雾化辅助接口的 HPLC/MS 在甘油三酯分析中的应用”质谱。

松本幸三: Anal.Sci.2. 3-7 (1986)

松本幸三：《肛门​​科学》2.3-7 (1986)

松本幸三: Mass Spectrometry. 35. 15-22 (1987)

松本幸三：质谱法。 35. 15-22 (1987)

Tadashi, TAGUCHI: "Application of Capillary Supercritical Fluid Chromatoguraphy to Oligomeric Polymer Analysis" International Symposium on Supercritical Fluids. October. 485-492 (1988)

Tadashi, TAGUCHI：“毛细管超临界流体色谱在低聚物分析中的应用”超临界流体国际研讨会。