Development of Directly Coupled Supercritical Fluid Chromatograph-Mass Spectrometer System

超临界流体色谱（SFC）是一种快速、高效的分离非挥发性和热不稳定性化合物的技术。由于超临界流体色谱的大部分移动的流动相如二氧化碳和正戊烷的挥发性比普通高效液相色谱高得多，因此，SEC与质谱仪直接联用（SFC-MS）在原理上是可行的。本课题将熔融石英填充毛细管柱的超临界流体色谱（SFC）与四极杆质谱仪（MS）通过真空雾化接口直接联用，成功地应用于各种非挥发性样品，如聚苯乙烯低聚物、非离子表面活性剂、邻苯二甲酸酯、甘油三酯聚乙二醇（PEG）衍生物和维生素。

Supercritical fluid chromatography(SFC) has been vigorously investigated and recognized as a rapid and powerful technique for separating non-volatile and thermally unstable compounds. Sine most of the mobile phases for SFC such as carbon dioxide and pentane have much higher volatility than those for ordinary HPLC, SEC is principally much feasible to make a direct coupling system with a mass spectrometr(SFC-MS). in this project, the SFC with the fused silica packed capillary column was directly coupled with a quadrupole MS through a vacuum nebulizing interface originally developed for HPLC-MS. Thus developed SFC-MS system was successfully applied to various non-volatile samples such as polystyrene oligomers, non-ionic surface agentswwhich are composed of complexed mixture of of origomeric polymers, phthalates, triglycerides, polyethylene glycol(PEG)derivatives and vitamins.