Investigation of the mechanisms of blue light-induced biological effects on skin and skin cells

蓝光对皮肤和皮肤细胞的生物效应机制研究

• 批准号: 403583563
• 负责人: Privatdozent Dr. Christian Opländer
• 金额: --
• 依托单位: Universitäres Herz- und Gefäßzentrum Hamburg (UHZ)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/403583563?language=en
• 关键词: Investigation; mechanisms; blue; light; induced

Recent studies have shown that apart from UV radiation also visible light exerts biological effects. For the first time, we could demonstrate that blue light (410, 420, 453 nm) affects proliferation of human dermal fibroblasts in a dose- and wavelength-dependent manner. In addition, irradiation with shorter wavelengths (410, 420 nm) dose-dependently induces cell toxicity, whereas non-toxic doses and wavelength of 453 nm are able to inhibit proliferation and reduce the antioxidative capacity. Moreover, we found an inhibition of TGF-beta1-induced differentiation of fibroblasts into myofibroblasts by blue light irradiation. As a possible mechanism for the observed effects, the blue light-induced generation of reactive oxygen species are assumed, however, neither the molecular mechanisms nor the participating signal pathways are sufficiently elucidated. Within the scope of this project, we want to further characterize blue light-induced effects on dermal fibroblasts, skin specimen and differentiation, and identify the involved mechanisms and pathways. Moreover, the efficacy of blue light treatment will be investigated in a 3D wound healing model an human skin specimen. For a possible clinical approach, safety aspects such as blue light-induced DNA damages or reduced function of keratinocytes coupled with a delay of re-epithelisation will be assessed. We expect interesting results from this project, which in turn may relevant for the treatment and prevention of Dupuytren contractures, hypertrophic scars, keloids and scleroderma as well as skin photoaging.

最近的研究表明，除了紫外线辐射外，可见光也会产生生物效应。这是第一次，我们可以证明，蓝光（410，420，453 nm）影响人皮肤成纤维细胞的增殖在剂量和波长依赖性的方式。此外，较短波长（410，420 nm）的照射剂量依赖性地诱导细胞毒性，而无毒剂量和453 nm波长能够抑制增殖并降低抗氧化能力。此外，我们发现了抑制TGF-β 1诱导的成纤维细胞分化成肌成纤维细胞的蓝光照射。作为所观察到的效应的可能机制，假定蓝光诱导产生活性氧，然而，无论是分子机制还是参与的信号通路都没有得到充分阐明。在本项目的范围内，我们希望进一步表征蓝光对真皮成纤维细胞，皮肤标本和分化的影响，并确定相关的机制和途径。此外，将在人体皮肤标本的3D伤口愈合模型中研究蓝光治疗的功效。对于可能的临床方法，将评估安全性方面，如蓝光诱导的DNA损伤或角质形成细胞功能降低伴上皮再形成延迟。我们期望从这个项目中得到有趣的结果，这反过来可能与治疗和预防Dupuytren挛缩，增生性瘢痕，瘢痕疙瘩和硬皮病以及皮肤光老化有关。