Initiation of starch granules and regulation of the starch granule number per chloroplast in Arabidopsis thaliana
拟南芥淀粉颗粒的引发和每个叶绿体淀粉颗粒数量的调节
基本信息
- 批准号:403800719
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2018
- 资助国家:德国
- 起止时间:2017-12-31 至 2022-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Over the last years the knowledge about starch metabolism, especially about the transitory starch metabolism in Arabidopsis thaliana, is tremendously increased. However, some fundamental questions are still open. Thus, how starch granules are initiated and which regulatory processed exist do determine the number of starch granules per chloroplast. This is also important for starch industrial applications and biotechnology. Recently, two Arabidopsis lines were identified which show a strong reduction in the number of starch granules in the chloroplast, ss4 and dpe2/phs1. Using extensive analyses we showed that more than one pathway of starch initiation exists. Furthermore, we showed that the number of starch granules can be altered by changes in the light/dark regime and by modification of the starch degradation process. Thus, these mutants are an excellent tool to analyse the process of starch initiation in more detail.This proposal uses various experimental approaches and focuses on the following aspects:1. Detailed analyses of PHS1 and DPE2 influences on starch granule formation, by altering the light regime, by alteration of the expression, by expression of inactive versions of these enzymes to distinguish between metabolic or structural functions of the enzymes, and by identify interaction partners of the proteins. 2. Analysis of the specific starch granule parameters3. Modify the starch degradation to identify the link between starch degradation and starch granule initiation.
近年来,人们对淀粉代谢,特别是拟南芥的瞬时淀粉代谢的认识有了很大的提高。然而,一些基本问题仍然悬而未决。因此,淀粉粒如何形成和调控过程的存在决定了每个叶绿体中淀粉粒的数量。这对淀粉工业应用和生物技术也很重要。最近,我们发现了两个叶绿体淀粉粒数明显减少的拟南芥品系,ss4和dpe2/phs1。通过广泛的分析,我们发现存在不止一种淀粉起始途径。此外,我们发现淀粉颗粒的数量可以通过光/暗制度的变化和淀粉降解过程的改变而改变。因此,这些突变体是更详细地分析淀粉起始过程的极好工具。本提案采用多种实验方法,重点关注以下几个方面:1。详细分析PHS1和DPE2对淀粉颗粒形成的影响,通过改变光照制度,通过改变表达,通过表达这些酶的无活性版本来区分酶的代谢或结构功能,并通过识别蛋白质的相互作用伙伴。2. 淀粉颗粒特性参数分析修改淀粉降解,以确定淀粉降解与淀粉颗粒形成之间的联系。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Professor Dr. Joerg Fettke其他文献
Professor Dr. Joerg Fettke的其他文献
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{{ truncateString('Professor Dr. Joerg Fettke', 18)}}的其他基金
Functional analysis of the starch related glucose 1-phosphate metabolism in higher plants
高等植物淀粉相关葡萄糖1-磷酸代谢的功能分析
- 批准号:
264514754 - 财政年份:2014
- 资助金额:
-- - 项目类别:
Research Grants
Regulation of the Activity and Location of Starch-Related Dikinases
淀粉相关二激酶活性和位置的调节
- 批准号:
184352434 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Research Grants
Deciphering carbohydrate/starch metabolisms in context of the oil yield in oil palm (Elaeis guineensis, Jacq.).
破译碳水化合物/淀粉代谢与油棕(Elaeis guineensis,Jacq.)油产量的关系。
- 批准号:
509434989 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
Detailed analyses of starch granule surface structure alterations
淀粉颗粒表面结构变化的详细分析
- 批准号:
417600839 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
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