Differential dopamine dynamics of DMS and DLS projecting SNc neurons during reversal learning

逆转学习过程中 DMS 和 DLS 投射 SNc 神经元的差异多巴胺动力学

• 批准号: 21K15184
• 负责人: RIOS・DAVILA ALAIN・ANTONIO
• 金额: $ 3万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K15184/
• 关键词: Dopamine; Basal Ganglia; Goal-directed behavior; dLight; Striatum; Basal ganglia; Goal directed behavior

Elucidation of the changes of the phasic activity of the DMS and DLS projecting SNc neurons, identified direct and indirect pathway striatal neurons, and local striatum dopamine release in rats performing a probabilistic reward-based task. We used TH-Cre, Drd2-Cre and Tac-1-Cre transgenic rats for dopaminergic neurons and striatal projecting neurons identification. For the investigation of dopamine release in dorsal striatum, we express dLight in DMS and DLS and monitored the fluorescence using fiber photometry.Elucidation of the differential role of intratelencephalic (IT) and corticofugal (CF) DMS corticostriatal OFC and mPFC in rats performing a probabilistic reward-based task. We use a viral vector approach for the identification of the different DMS corticostriatal neurons.

阐明的DMS和DLS投射SNc神经元的阶段性活动的变化，确定直接和间接通路纹状体神经元，和局部纹状体多巴胺释放大鼠执行概率奖励为基础的任务。我们使用TH-Cre、Drd 2-Cre和Tac-1-Cre转基因大鼠进行多巴胺能神经元和纹状体投射神经元的鉴定。为了研究背侧纹状体多巴胺的释放，我们表达dLight在DMS和DLS和监测荧光使用fiber photometrics.Elucidation的端脑内（IT）和离皮质（CF）DMS皮质纹状体OFC和mPFC在大鼠执行概率奖励为基础的任务的差异作用。我们使用病毒载体的方法来识别不同的DMS皮质纹状体神经元。

项目成果

Dynamic and pathway-specific signaling in the dorsomedial striatum and dopamine neurons during behavioral adaptation

行为适应过程中背内侧纹状体和多巴胺神经元的动态和通路特异性信号传导

• 发表时间: 2022
• 作者: Rios Alain