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Neural mechanism underlying cognitive operations in emotional learning systems

情绪学习系统中认知操作的神经机制

基本信息

批准号：
21K15212
负责人：
GU XIAOWEI
金额：
$ 2.91万
依托单位：
Institute of Physical and Chemical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K15212/
关键词：
emotional learning mPFC amygdala fear inference rodent

项目摘要

In this year, the project had significant progress. I finished all the data collection of calcium imaging during the behavioral experiments of rats. I also analyzed the recorded data by binary decoding, statistics calculation, PCA trajectory and so on. I found that neurons in prelimbic area significantly responded to conditioned sensory stimulus indicating inferred fear memory and original fear memory. Compared with rats without inferred fear memory, rats with fear inference had more neurons responding to both conditioned sensory stimulus indicating inferred fear memory and original fear memory. These co-responsive neurons may play important role in the integration of aversive memory and associated inferred memory. At the same time, prelimbic neurons projecting to amygdala showed more specific response to conditioned stimulus indicating inferred fear memory rather than original fear memory, which is consistent with previous optogenetics results. When the relationship between inferred memory and aversiveness being dissociated, there was reduction of neurons responding to both unconditioned stimulus and sensory stimulus. All these results illustrate the mechanism of cognitive system of rats brain manipulate the formation of inferred emotional expression. The results were valuable to develop new therapy for aversiveness disorder in human.

今年，该项目取得了重大进展。完成了大鼠行为学实验中钙显像的所有数据采集。通过二进制解码、统计学计算、PCA轨迹等方法对记录的数据进行分析，发现边缘前区神经元对条件感觉刺激有明显的反应，提示推断恐惧记忆和原始恐惧记忆。与无推断恐惧记忆的大鼠相比，有推断恐惧记忆的大鼠有更多的神经元对暗示推断恐惧记忆和原始恐惧记忆的条件感觉刺激作出反应。这些共反应神经元可能在厌恶记忆与联想推断记忆的整合过程中发挥重要作用。同时，投射到杏仁核的前边缘系统神经元对条件刺激表现出更特异的反应，表明推断的恐惧记忆而不是原始的恐惧记忆，这与先前的光遗传学结果一致。当推断记忆与厌恶性的关系被分离时，对非条件刺激和感觉刺激的反应神经元均减少。这些结果说明了大鼠脑认知系统操纵推断性情绪表达形成的机制。本研究结果对开发人类厌恶性障碍的新疗法有一定的参考价值。