Investigation on the induction mechanism of hepatocyte death by fungal reactive oxygen species

白念珠菌CaCFL11在与肝细胞共培养下表达增强，导致ROS产生并引起肝细胞凋亡现象。我们构建了CaCFL 11基因启动子与GFP融合的杂合突变体。这些突变体的结果表明，CaCFL 11的表达在与肝细胞共培养时受到调节，并有助于靶向参与CaCFL 11表达的转录因子的结合区域。另一方面，天然产物姜黄素已被广泛研究作为一种药物候选人，并表现出抗真菌活性对C。白色念珠菌本实验室抗真菌药物耐药性研究表明，姜黄素影响了热休克蛋白90（Hsp90）的功能和多药转运蛋白Cdr 1的药物外排泵活性。白色念珠菌

Candida albicans CaCFL11 showed enhanced expression under the co-culture with hepatic cells, resulting in ROS production and cause hepatocytes apoptosis phenomenon. We constructed heterozygous mutants of CaCFL11 gene promoter fused to GFP. The results from these mutants indicated CaCFL11 expression was regulated in response to co-culture with hepatocytes and helped to target the binding region of transcription factors involved in the CaCFL11 expression. On the other hand, the natural product curcumin has been widely studied as a drug candidate and exhibited antifungal activity against C. albicans. Our study on antifungal drug resistance showed curcumin affected the function of heat shock protein 90 (Hsp90) and drug efflux pump activity of multidrug transporter protein Cdr1 in C. albicans.