新生児慢性肺疾患におけるピルビン酸デヒドロゲナーゼ4の重要性

丙酮酸脱氢酶 4 在新生儿慢性肺病中的重要性

• 批准号: 21K15877
• 负责人: 伊藤 誠人
• 金额: $ 3万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K15877/
• 关键词: PDK4; 気管支肺異形成; 新生児慢性肺疾患

・Pdk4 KOおよび野生型肺における各種炎症性サイトカインのmRNA測定についてPdk4 KOおよび野生型マウスを生後96時間酸素暴露（95%）をおこない日齢4，日齢14で解剖を行い肺を摘出して、mRNA測定を行った。野生型マウスは酸素暴露下で日齢4においてIL6、MCP1が上昇し、日齢14でも持続しているのに比して、Pdk4 KOマウスは日齢4，日齢14ともにIL6、MCP1の上昇を認めなかった。・新生仔期に高濃度酸素を暴露させたPdk4 KOマウスの病理像の変化について同様にPdk4 KOおよび野生型マウスを生後96時間酸素暴露（95%）をおこない日齢4，日齢14で解剖を行い肺を摘出して評価を行った。日齢４における酸素暴露後の肺胞拡大、二次中隔減少は野生型マウス,PDK4ノックアウトマウスともにみられるが日齢14（回復期）にはPDK4　ノックアウトマウスのほうが肺胞径、二次中隔数とも有意差をもって改善が見られた。・Polymorphism and chronic lung disease in preterm infants現在4施設より210検体を集め、DNA抽出を行い、PDK4の一塩基多型について調査中である。

Pdk4 KO was used to determine the level of serum alanine at 96 hours after birth (95%) and 4 days after birth in each group of inflammatory lung cancer in each group of inflammatory lung cancer. MRNA test was performed on 14 days after birth. MRNA test was performed. IL6, MCP1, Pdk4 KO, IL6 and MCP1 were exposed to acid exposure on the 4th day and 14th day, respectively. The histopathology of neonatal Pdk4 KO exposure was similar to that of Pdk4 KO exposure (95%) at 96 hours after birth, and the lungs were dissected on the 14th day after birth. After 4 days of acid exposure, the lung cells were enlarged, the secondary septum was reduced, and the wild type was PDK4. The lung cell diameter, secondary septum, lung cell diameter, lung diameter, lung cell diameter and secondary septum were measured. The lung cell diameter, secondary septum, lung cell diameter and secondary septum were Polymorphism and chronic lung disease in preterm infants is currently in the 4-year implementation of the 210th model collection, the DNA pull-out line, and the PDK-4 single-base multi-model model.