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革新的な乳房再建術のための脂肪由来幹細胞と生体外増幅単核球の相互作用の解析

分析脂肪干细胞与体外扩增的单核细胞之间的相互作用，用于创新的乳房重建手术

基本信息

批准号：
21K16919
负责人：
古川聖美
金额：
$ 3万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2021
资助国家：
日本
起止时间：
2021-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

本研究は、乳がん患者乳房切除後の乳房再建成績向上による患者QOL改善のため、血管再生が得意な生体外増幅単核球と脂肪再生が得意な間葉系幹細胞を混合することにより、生着率の高い脂肪移植技術を開発することを目的としている。昨年度までの研究により、生体外増幅単核球(RE01細胞)と共培養することで、脂肪由来間葉系幹細胞(ASC)の脂肪分化能や　血管形成能が向上することがin vitro実験において判明している。そこで、本年度はヌードマウスの背部移植実験を実施し、脂肪移植における有効性を検討した。ヒト脂肪組織200μLにASC 2x10^4 cellを混合し、ヌードマウス背部に移植し、体積の経時変化をCTにより検証した。また、移植4週間後に移植片を採取し、組織標本を作製した。微小血管数計測のため、抗CD31抗体、生脂肪細胞計測のため抗Perillipin抗体、炎症細胞計測のため抗iNOS抗体を用いて免疫染色を実施し、移植脂肪組織の解析を行った。細胞なしのコントロール群および共培養をしていない通常のASC混合群と比較し、RE01刺激ASC混合移植群において、移植脂肪の体積が優位に維持されていた。また、組織解析の結果、移植脂肪組織中の血管数や脂肪の質もRE01刺激ASC混合群にて優位に促進していた。逆に炎症反応は減少していた。本年度の動物実験の結果から、RE01と共培養したASCの脂肪移植における有効性が示された。

In this study, breast reconstruction was performed after mastectomy in patients with breast cancer, QOL improvement, angiogenesis, in vitro nuclear globule fat regeneration, high fat transplantation, high fat transplantation. Last year, RE01 cells were co-cultured in vitro, and the origin of fat was known as ASC. The differentiation of fat could lead to the formation of blood vessels. In vitro cells showed that blood vessels could be formed. This year, this year, we will have a back transplant, a fat transplant, and a fat transplant. The fat tissue was mixed with 200 μ L ASC 2x10 ^ 4 cell, the back was implanted, and the body was temporarily modified into CT. After 4 years of transplantation, the grafts were collected and the tissue tags were used. Microvessel count, anti-CD31 antibody, adipocytic cell count, anti-Perillipin antibody, inflammatory cell count, anti-iNOS antibody were detected by immunostaining, and adipose tissue grafts were analyzed. We usually use ASC mixed group to compare, RE01 stimulate ASC mixed transplant group to compare, and fat grafts to maintain the position of fat grafts. The number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted adipose tissue, the number of blood vessels in the transplanted Anti-inflammation, anti-inflammation, anti-inflammation and anti-inflammation. The results of this year's animal experiments show that RE01 co-cultured ASC fat transplants to show sexual signs.