Development of technology to control osteogenic differentiation of iPS cells using shear-stress loading

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1. Shear stress (0.05,0.5, and 1.5 Pa) enhanced osteogenic differentiation ofiPSCs compared to the static group2. 0.5 Pa induced mesoderm and endoderm differentiation of iPSCs while suppressingectoderm differentiation. Among mesodermal derivatives, endothelial cell markers were markedly upregulated by shear stress.3. 0.5 Pa suppressed OPG gene expression but interestingly enhanced the release of OPG by osteogenically-induced iPSCs. In contrast, 0.5 Pa also enhanced RANKL gene and protein expressions.