Development of technology to control osteogenic differentiation of iPS cells using shear-stress loading

开发利用剪切应力负载控制 iPS 细胞成骨分化的技术

• 批准号: 21K17005
• 负责人: Limraksasin Phoonsuk
• 金额: $ 3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K17005/
• 关键词: iPS cells; shear force loading; bone tissue engineering; bone regeneration; Bone regenration; shear stress

1. Shear stress (0.05,0.5, and 1.5 Pa) enhanced osteogenic differentiation ofiPSCs compared to the static group2. 0.5 Pa induced mesoderm and endoderm differentiation of iPSCs while suppressingectoderm differentiation. Among mesodermal derivatives, endothelial cell markers were markedly upregulated by shear stress.3. 0.5 Pa suppressed OPG gene expression but interestingly enhanced the release of OPG by osteogenically-induced iPSCs. In contrast, 0.5 Pa also enhanced RANKL gene and protein expressions.

1.与静止组相比，剪切力(0.05、0.5和1.5pa)促进了PSCs的成骨分化。0.5pA诱导IPSCs中胚层和内胚层分化，抑制外胚层分化。在中胚层衍生物中，内皮细胞标记物在剪切力作用下明显上调。0.5pA抑制成骨诱导的IPSCs的OPG基因表达，但促进OPG的释放。相反，0.5pA也促进了RANKL基因和蛋白的表达。