Hybridization kinetics and non-equilibrium dynamics of random pools of oligonucleotides (P15)

我们将从实验和理论上研究寡核苷酸池中的杂交动力学，并调查池的组成--单链、部分杂交、非同源复合体和“完全匹配”的复合体--如何随着时间的推移而变化。由于杂交强烈依赖于环境参数，如盐浓度和pH，因此我们将研究盐梯度内层序库的时空动力学。我们将开发实验方法，在不同的地点监测池塘中的不同物种，并开发理论工具来分析这些复杂的动态。

We will study, both experimentally and theoretically, hybridization kinetics within oligonucleotide pools and investigate how the composition of the pool – single-strands, partially hybridized, non-cognate complexes, and “perfectly matched” complexes – changes over time. As hybridization is strongly dependent on environmental parameters such as salt concentration and pH, we will then study the spatiotemporal dynamics of the sequence pools within salt gradients. We will develop experimental methods to monitor different species within the pool at different locations, and theoretical tools to analyze these complex dynamics.