Elucidating roles of Wnt gradients and PCP signaling in establishing neural polarity during column formation

阐明 Wnt 梯度和 PCP 信号在柱形成过程中建立神经极性中的作用

• 批准号: 22K15121
• 负责人: WANG Miaoxing
• 金额: $ 2.91万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15121/
• 关键词: Wnt/PCP; column formation; Wnt; PCP; neuron

Wnt/PCP signaling is evolutionarily conserved from Drosophila to humans and is highly related to cancer and Alzheimer’s disease. Nevertheless, recent studies argue contradictory results about the roles of Wnt ligands in regulating PCP signaling. Our study utilizes an excellent model system to clarify this problem by revealing evolutionarily conserved mechanisms of column formation.In this project, we have generated knock-in alleles to visualized subcellular distribution of PCP proteins; 2) we identified the subcellular distribution of PCP proteins in the singal core columnar neurons by combining specific neurons-FLPase strains that express FLPase specifically in core columnar neurons with the knock-in strains. These findings and results provide an important basis for our research.

Wnt/PCP信号转导在果蝇和人类之间是进化保守的，并且与癌症和阿尔茨海默病高度相关。然而，最近的研究认为Wnt配体在调节PCP信号传导中的作用是相互矛盾的。我们的研究利用一个很好的模型系统来阐明这个问题，通过揭示柱形成的进化保守机制，在这个项目中，我们已经产生了敲入等位基因来可视化PCP蛋白的亚细胞分布; 2）我们通过将在核心柱状神经元中特异性表达FLP 3的特异性神经元-FLP 3菌株与敲除的FLP 3菌株相结合，在菌株中。这些发现和结果为我们的研究提供了重要依据。