甲状腺がん未分化転化に至る形態学的構造の分子病理学的in situ解析

甲状腺癌间变性转化形态结构的分子病理原位分析

• 批准号: 22K15407
• 负责人: ムサジャノワ ジャンナ
• 金额: $ 2.91万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K15407/
• 关键词: 甲状腺がん; 未分化がん; 細胞接着; 極性; 上皮間葉転換

甲状腺乳頭がんのHobnail亜型は再発や遠隔転移の頻度が高く予後不良で、未分化転化と関係し原病死につながる。Hobnailパターンの形態学的特徴は、がん細胞の極性の乱れと細胞接着の異常（LOP/LCC）を反映し、転移・浸潤能の獲得につながる上皮―間葉転換（EMT）を示唆する。97例の原発性甲状腺がんを解析し、16例のBRAFV600EとTERT promoterのdouble mutantを検出し、その全てで浸潤部にLOP/LCCパターンを認め、14例がpT3 or 4、10例がpN1で、9例がKi-67標識率6% 以上であることが判明した。これらを対象とし、通常型乳頭がん成分では正常濾胞と比較してCK8/18とClaudin-1発現が亢進し、VimentinとZO-1発現の極性（Apical-basal polarity）は保持され、予後不良亜型である高細胞亜型にはβ-cateninの異所性発現（核上部胞体内発現）やClaudin-1とVimentin不均一発現が、Hobnail亜型成分にはVimentin, Claudin-1, ZO-1、β-cateninの発現低下が関与し、さらに低分化がんにはZO-1発現は保たれApical-basal polarityは維持されているが、CK8/18とVimentinの発現が消失することが判明した。これらの結果は、甲状腺がん進行に関連する組織構築の変化過程には細胞骨格と細胞間接着のダイナミックな変化が関与することを示唆していて、細胞接着性や中間フィラメントの調節異常が高分化がんの予後不良亜型や低分化がんの形態形成に重要と思われる。

The frequency of recurrence and distant migration of thyroid papilla is high, and the relationship between poor differentiation and death is high. The morphological characteristics of Hobnail's skin include: cell polarity disorder, abnormal cell adhesion (LOP/LCC), epithelial mesenchymal transition (EMT), and migration and infiltration. 97 cases of primary thyroid were analyzed, 16 cases of BRAFV600E and TERT promoter were detected, all cases of LOP/LCC were detected, 14 cases of pT 3 or 4, 10 cases of pN 1, 9 cases of Ki-67 were detected, and the identification rate was more than 6%. The normal nipple component is opposite to the normal filter cell. The CK 8/18 Claudin-1 appears opposite to the normal nipple component. The Vimentin ZO-1 appears opposite to the normal filter cell.(Apical-basal polarity) is maintained, and after the bad type, the high cell type is the β-catenin and the heterosexual development.(In the upper part of the nucleus) Claudin-1 and Vimentin heterogeneously occur, Hobnail-type components Vimentin, Claudin-1, ZO-1, β-catenin occur less frequently, ZO-1 occur less frequently, Apical-basal polarity remains less frequently, Hobnail-type components occur less frequently, ZO-1 occur less frequently, Apical-basal polarity remains less frequently, Hobnail-type components occur less frequently, Hobnail-type components occur less frequently, ZO-1 occur less frequently, ZO-1 occur less frequently, Apical-basal polarity remains less frequently, Hobnail-type components occur less frequently, Hobnail-type components occur less frequently, ZO-1 occur less frequently, ZO-1 occur less frequently, Apical-basal polarity remains less frequently, Hobnail-type components occur less frequently, Hobnail-type components occur less frequently, Hobnail-type components occur less frequently, ZO-1 occur less frequently, ZO-type components occur less frequently, ZO-1 occur less frequently, Apical-basal polarity remains less frequently, Hobnail-type components occur less frequently, Hobnail CK 8/18: Vimentin's appearance disappeared. As a result, the thyroid gland is related to the process of tissue architecture, cell bone, cell adhesion, and cell differentiation. The regulatory abnormalities of cell adhesion and cell adhesion are important for the formation of high differentiation and poor differentiation.

项目成果

充実性成分を呈する結節内結節型甲状腺結節の分子病理学的特徴解析

具有实性成分的结节内结节甲状腺结节的分子病理学特征

• 发表时间: 2022
• 作者: 松田勝也;上田真由;Mussazhanova Zhanna;Saiaubekova Yerkezhan;黒濵大和;松岡優毅;大坪智恵子;佐藤伸也;山下弘幸;中島正洋
• 通讯作者: 中島正洋

低分化成分を有する結節内結節型甲状腺良性結節の分子病理学的特徴解析

低分化良性甲状腺结节的分子病理学特征

• 发表时间: 2022
• 作者: 上田真由;松田勝也;黒濵大和;Mussazhanova Zhanna;Yerkezhan Sailaubekova;近藤久義;松岡優毅;大坪智恵子;佐藤信也;山下弘幸;川上 純;中島正洋
• 通讯作者: 中島正洋

Molecular Pathological Characteristics of Benign Thyroid Nodules with Poorly Differentiated Component

低分化良性甲状腺结节的分子病理特征

• 发表时间: 2022
• 作者: Ueda M;Matsuda K;Kurohama H;Mussazhanova Z;Sailaubekova Y;Kondo H;Matsuoka Y;Otsubo C;Sato S;Yamashita H;Kawakami A;Nakashima M
• 通讯作者: Nakashima M

低分化成分を有する非浸潤性甲状腺結節の分子病理学的特徴解析

低分化非侵袭性甲状腺结节的分子病理学特征

• 发表时间: 2022
• 作者: 上田真由;松田勝也;黒濵大和;Mussazhanova Zhanna;Yerkezhan Sailaubekova;近藤久義;松岡優毅;大坪智恵子;佐藤信也;山下弘幸;川上 純;中島正洋
• 通讯作者: 中島正洋

A new indicator to differentiate thyroid follicular inclusions in the cervical lymph nodes from patients with thyroid cancer

区分颈部淋巴结中甲状腺滤泡包涵体与甲状腺癌患者的新指标

• 发表时间: 2022
• 作者: Mussazhanova Z;Otsubo C;Kurohama H;Shalgimbayeva G;Ueki N;Matsuoka Y;Madiyeva M;Sato S;Yamashita H;Nakashima M
• 通讯作者: Nakashima M