权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Elucidating the role of extracellular vesicles microRNA cargo in HIV associated immune dysfunction

阐明细胞外囊泡 microRNA 货物在 HIV 相关免疫功能障碍中的作用

基本信息

批准号：
22K16374
负责人：
BARABONA GODFREY
金额：
$ 2.91万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K16374/
关键词：
Extracellular vesicle HIV Inflammation MicroRNA

项目摘要

Our project had two main specific aims: (1) to identify circulating miRNAs that are altered in HIV-infected individuals, and (2) to investigate whether altered miRNAs in extracellular vesicles from HIV patients regulate cytokine expression ex vivo. Over the past year of project implementation, we have collected three sets of plasma samples: from HIV-infected individuals before and after treatment, and from HIV-uninfected individuals. Using these samples, we have demonstrated a differential relative expression of miRNAs in plasma extracellular vesicles (EVs). Notably, our preliminary data suggest a trend of downregulation of EV miRNAs in the untreated HIV-infected group compared to the treated and HIV-uninfected groups. Our extended analysis further indicates that absolute levels of miRNA are lower in HIV-infected untreated individuals compared to treated and uninfected individuals. Additionally, we found that plasma miRNAs were more abundant compared to miRNAs in EVs but followed a similar trend to that of EVs. Given that absolute levels of miRNAs seem to be low in most individuals, we intend to first demonstrate the effect of EV miRNAs from HIV-infected individuals compared to those from HIV-uninfected individuals in vivo.

我们的项目有两个主要的特定目标：(1)确定HIV感染者体内循环中改变的miRNAs，以及(2)研究HIV患者细胞外小泡中改变的miRNAs是否在体外调节细胞因子的表达。在项目实施的过去一年中，我们收集了三组血浆样本：艾滋病毒感染者治疗前和治疗后的血浆样本，以及艾滋病毒未感染者的血浆样本。使用这些样本，我们已经证明了miRNAs在血浆细胞外小泡(EV)中的不同相对表达。值得注意的是，我们的初步数据表明，与接受治疗和未感染艾滋病毒的组相比，未经治疗的艾滋病毒感染者组中肠道病毒miRNAs的表达有下调的趋势。我们的扩展分析进一步表明，与接受治疗和未感染的人相比，感染艾滋病毒的未经治疗的人的miRNA绝对水平更低。此外，我们发现血浆miRNAs在EVS中比miRNAs更丰富，但遵循与EVS相似的趋势。鉴于大多数人体内miRNAs的绝对水平似乎很低，我们打算首先证明来自艾滋病毒感染者的肠道病毒miRNAs与来自HIV非感染者的肠道病毒miRNAs在体内的影响。