歯周炎によるNAFLD増悪における腸管バリア機能の関与の解明

阐明肠道屏障功能在牙周炎引起的 NAFLD 恶化中的作用

• 批准号: 22K17039
• 负责人: 田利 美沙子
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K17039/
• 关键词: 歯周炎によるNAFLD増悪; 歯周炎; NAFLD; 腸管バリア機能

高炭水化物食HCDを投与したマウスの上顎第2大臼歯に5-0絹糸を結紮することで、NAFLD増悪歯周炎モデルとした。対照群として、普通食(NC: Normal chow)投与群、NC投与+絹糸結紮群、HCD投与群を設け、前述した予備的検討からエンドポイントを実験開始6週とし、4週、6週で経時的に評価した。4週において、高炭水化物食投与群のうち、絹糸結紮群で非絹糸結紮群と比較して肝臓の脂肪変性の増悪、線維化、炎症性細胞の浸潤、肝細胞の風船化等NAFLD増悪と考えられる所見を認めた。また、普通食投与群においても、絹糸結紮群で非絹糸結紮群と比較して、多くの個体で脂肪変性を認めた。各実験群から腸管上皮を回収し、H-E染色を行った。普通食投与群、高炭水化物食投与群のいずれにおいても、絹糸結紮群で、非絹糸結紮群と比較して、粘膜上皮の萎縮を認めた。炎症性変化の肺細胞数の変化はみられなかった。一方で、各実験群から直腸を採取し、real-time PCRで炎症性サイトカインの発現を比較したところ、各実験群間に有意な差は認めなかった。各実験群から肝臓を回収・破砕して血液寒天培地に播種し細菌コロニー形成を行った。各実験群において、細菌のコロニー形成は認めなかった。また、腸管上皮と同様にreal-timePCRを行い炎症性サイトカインの発現を調べたが、IL-1β、IL-6、TNF-αにおいて発現に有意差は認めなかった。

The high-carbon hydrate HCD was added to the diet of high-carbon hydrates, and the second acetabular was 5-0. The results showed that the high-carbon hydrates were mixed with high-carbon hydrates, and the high-carbon hydrate was added to the diet of high-carbon hydrates, HCD and NAFLD. According to group food, general food (NC: Normal chow), NC investment and group, HCD investment and group settings, and the aforementioned one, the six-month-old, four-and six-year-old start-up of the six-month-old, four-month-old and six-week cycle. (4) the results of NAFLD tests, such as high-carbon diet, high-carbon hydrates, non-series, non-series, liver fat, inflammatory cell immersion, liver cytophagy, etc. The results of general food intake, general diet, general diet and general diet were compared. In each group, the epithelium of the tube was detected, and Hmure E staining was performed. General food intake and group, high-carbon hydrate diet and group diet, non-diet group, high carbon hydrate group and high carbon hydrate group. Inflammatory inflammation, lung cancer, lung cancer and lung cancer. In the same group, all the groups were collected directly, and the results of real-time PCR were significantly higher than those in the control group. In each population, the liver was broken and the blood was broken. In cold weather, the earth was planted with bacteria to form a drug. Each group of bacteria and bacteria formed a small group of bacteria. The epithelium of the tube and real-timePCR were the same as those of the inflammatory group. We found that the expression of IL-1 β, IL-6, TNF- α was significantly different from that of the control group.