炎症収束脂質メディエーターのレゾルビンD2の硬組織誘導能のメカニズムの検討

消炎素 D2（一种炎症聚合脂质介质）诱导硬组织能力的机制研究

• 批准号: 22K17058
• 负责人: 岡本 憲太郎
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K17058/
• 关键词: 直接覆髄法; RvD2; 根尖性歯周炎; 硬組織誘導; レゾルビンD2; 炎症抑制

う蝕や外傷によって露髄した歯の治療には，多くの場合で抜髄処置が選択されてきた。近年，硬組織誘導能に優れた覆髄材料や，封鎖性に優れた覆髄材料が開発され，歯髄組織を保護して歯の生存率を伸ばすことを目的とした直接覆髄法に注目が集まっている。今回，新規の直接覆髄剤として炎症収束脂質メディエーターのレゾルビンD2（RvD2）に着目した。これまでにSiddiquiらはラット根尖性歯周炎モデルにおいて，RvD2が抗炎症作用と根尖部での硬組織形成を促進して，根尖性歯周炎の治癒を誘導することを報告した。そこで本研究では，RvD2が抗炎症作用と硬組織形成作用を発揮することで，既存の直接覆髄剤とは異なったメカニズムによって，直接覆髄法における良好な治療結果をもたらすのではないかと仮説を立てた。そして，ラット断髄モデルを用いて，RvD2の歯髄組織への影響をin vivoで検討した。さらに，その詳細な免疫学的なメカニズムを，歯髄細胞を用いてin vitroで検討した。1.新生象牙質様硬組織の観察：ラット断髄モデルで，断髄面にRvD2高濃度・低濃度，水酸化カルシウム製剤もしくはPBSを塗布し窩洞封鎖した。4週および6週後に断髄面に形成された新生象牙質様好組織を動物用マイクロCTで観察した。2.ラット歯髄組織の組織化学的解析：ラット断髄モデルで得たサンプルから組織切片を作製しRvD2受容体であるGPR18の歯髄組織における局在を，蛍光免疫染色を行って観察した。3.細胞増殖活性の評価：歯髄細胞に対するRvD2の細胞増殖活性を調べた。歯髄細胞を96-wellマルチプレートに播種し，培養細胞の接着面が80％程度のサブコンフルエント状態になるまで培養した。その後，RvD2（最終濃度1，10，100 nM）または培地を添加し，24時間後にCell Counting Kit-8を用いて，細胞増殖活性の評価を行った。

Please do not know how to treat the disease. If you do not want to do so, you will need to select the appropriate information. In recent years, the hardware organization has been able to conduct a comprehensive review of the materials, and the sealing materials have been used to protect the survival rate and the survival rate of the disease. In recent years, the direct coverage method has focused on the protection of critical survival rates. This time, the new regulations are directly covered by inflammation, inflammation, fat, fat and fat. The treatment of periapical periodontitis caused by Siddiqui, the anti-inflammatory effect of RvD2, the promotion of hard tissue formation at the apical, and the treatment of periapical periodontitis. In this study, the anti-inflammatory effect of RvD2, the formation of hard tissue, the formation of hard tissue, the effect of anti-inflammatory, the formation of hard tissue, the effect of anti-inflammatory, the formation of hard tissue, the effect of anti-inflammatory, the formation of hard tissue, the effect of anti-inflammatory, the formation of hard tissue, the effect of anti-inflammation, the formation of hard tissue, the effect of anti-inflammation, the formation of hard tissue, the effect of anti-inflammation, the formation of hard tissue, the effect of anti-inflammation, the effect of hard tissue formation, the formation of hard tissue, the effect of anti-inflammatory tissue formation. In this case, you will not be able to use the information, and the RvD2 organization will have an impact on the in vivo. The immunology of immunology is called "in vitro" and "cell" is used for immunology. 1. The hard tissue of the newborn ivory was observed: the tooth was cut off, the fracture surface was RvD2 high and low, and the water acidification was used to seal the holes in the PBS. 4. After 6 weeks, the new ivory was formed on the broken surface. The tissue was well organized and the animals were examined by scanning CT. two。 Analysis of tissue chemistry: the tissue sections of RvD2 recipients were detected by light immunostaining, and the tissue sections were examined by light immunostaining. 3. Cell colonization activity: cell colonization activity: cell colony activity. RvD2 activity. In 96-well, the seeds are sown, and then the cells are exposed to 80% of the temperature, and the status is improved. After incubation, RvD2 (maximum temperature 10100 nM) was added to the culture ground, and Cell Counting Kit-8 was used after 24 hours, and the colonization activity was tested.